TY - JOUR AB - Sirtuins are critical players within multiple cellular pathways such as stress response, apoptosis and energy metabolism. They are associated with metabolic and degenerative diseases, the pathogenesis of cancer and are key elements in the regulation of cellular life span. From within the 7 known human sirtuins, SIRT3 recently stepped out of the shadow of SIRT1 showing strong effects on stress response, apoptosis, cell cycle and energy metabolism, mimicking effects of caloric restriction. We have identified two non-synonymous human SIRT3 SNPs and evaluated their impact on SIRT3 activity and stability. We assessed their influence on cellular energy metabolism in relation to SIRT1 and identified SIRT3 to increase cellular respiration by 80% when compared to SIRT1, which increased cellular respiration by only 30%. AD - Saarland University Medical Center, Department of Internal Medicine, Division of Immunotherapy and Gene Therapy, José Carreras Research Center, D-66421 Homburg/Saar, Germany null AU - Dransfeld,Christian-Lars AU - Alborzinia,Hamed AU - Wölfl,Stefan AU - Mahlknecht,Ulrich DA - 2010/04/01 DO - 10.3892/ijo_00000574 EP - 960 IS - 4 JO - Int J Oncol PY - 2010 SN - 1019-6439 1791-2423 SP - 955 ST - SIRT3 SNPs validation in 640 individuals, functional analyses and new insights into SIRT3 stability T2 - International Journal of Oncology TI - SIRT3 SNPs validation in 640 individuals, functional analyses and new insights into SIRT3 stability UR - https://doi.org/10.3892/ijo_00000574 VL - 36 ER -