TY - JOUR AB - Clear cell sarcoma (CCS) affects the deep soft tissues in young adults and is known to have high rates of metastasis, including lymphatic metastasis. In our previous study an xenoplant model of CCS was established, which exhibited local tumor growth, lymphatic metastasis, and distant metastasis in SCID‑Beige mice. In the current study, the role of NK cells during metastasis in the same xenoplant murine model was investigated. Injection of murine or human NK cells significantly suppressed the metastasis of HS‑MM CCS cells in SCID‑Beige mice. Notably, reverse transcription‑quantitative PCR analysis demonstrated that injection of NK cells did not alter the mRNA expression levels of ERSR1‑ATF1, which is specifically transcribed in CCS, in the buffy coat of circulating blood cells of HS‑MM‑xenoplanted SCID‑Beige mice. BALB/c nude mice xenoplanted with HS‑MM cells exhibited local growth without evident metastasis, whereas inoculation with the anti‑asialo‑GM1 antibody, which has previously been found to abolish NK‑cell activity, resulted in metastasis of HS‑MM cells in BALB/c nude mice. The injection of the anti‑CD96 antibody, which increases the cytotoxicity of NK cells, significantly suppressed the metastasis of HS‑MM cells in SCID‑Beige mice. These results indicated that NK cells impaired the metastatic tumor microenvironments in the present mice xenoplant model. AD - Department of Pathology and Translational Research, Gifu University Graduate School of Medicine, Gifu 501‑1193, Japan AU - Hanamatsu,Yuki AU - Saigo,Chiemi AU - Kito,Yusuke AU - Takeuchi,Tamotsu DA - 2021/01/01 DO - 10.3892/mco.2020.2171 IS - 1 JO - Mol Clin Oncol KW - metastatic tumor microenvironment clear cell sarcoma metastatic model NK cells ERSR1‑ATF1 PY - 2021 SN - 2049-9450 2049-9469 SP - 9 ST - An obstructive role of NK cells on metastatic growth of clear‑cell sarcoma cells in a xenoplant murine model T2 - Molecular and Clinical Oncology TI - An obstructive role of NK cells on metastatic growth of clear‑cell sarcoma cells in a xenoplant murine model UR - https://doi.org/10.3892/mco.2020.2171 VL - 14 ER -