TY - JOUR AB - Breast cancer (BC) is common worldwide. c‑Myc and AXL are both overexpressed in BC, promoting its progression. The present study aimed to investigate the role of AXL in c‑Myc expression in BC. Overexpression of AXL increased c‑Myc expression while knockdown of AXL decreased c‑Myc expression as determined by western blot analysis. Pharmaceutical inhibition of AXL also suppressed c‑Myc expression. AKT and ERK inhibitor LY294002 and U0126 suppressed c‑Myc expression, respectively. AXL overexpression which activates AKT and ERK signaling, upregulates c‑Myc expression, while kinase‑dead AXL which cannot activate AKT and ERK signaling, does not upregulate c‑Myc expression, emphasizing the important role of these two signaling pathways in c‑Myc upregulation. Finally, expression data of BC tissues from The Cancer Proteome Atlas displayed an association between AXL and c‑Myc. Taken together, the present study revealed that AXL upregulates c‑Myc expression through AKT and ERK signaling pathways in BC. AD - Department of Pathology, Jinan Adicon Clinical Laboratory, Jinan, Shandong 250000, P.R. China Clinical Laboratory, The Third People's Hospital of Jinan, Jinan, Shandong 250132, P.R. China College of Basic Medicine, Jining Medical University, Jining, Shandong 272067, P.R. China Institute of Forensic Medicine and Laboratory Medicine, Jining Medical University, Forensic Science Center of Jining Medical University, Jining, Shandong 272067, P.R. China Department of Digestive System, Shandong Institute of Parasitic Diseases, Shandong First Medical University and Shandong Academy of Medical Sciences, Jining, Shandong 272067, P.R. China Department of Hematology, Jinan Children's Hospital, Jinan, Shandong 250132, P.R. China AU - Sun,Xiaobai AU - Chen,Hong AU - You,Shuling AU - Tian,Zhikang AU - Wang,Zhaoyu AU - Liu,Fulin AU - Hu,Wenyi AU - Zhang,Hao AU - Zhang,Guoan AU - Zhao,Hongli AU - Guo,Qingwei DA - 2023/03/01 DO - 10.3892/mco.2023.2618 IS - 3 JO - Mol Clin Oncol KW - AXL c‑Myc breast cancer PY - 2023 SN - 2049-9450 2049-9469 SP - 22 ST - AXL upregulates c‑Myc expression through AKT and ERK signaling pathways in breast cancers T2 - Molecular and Clinical Oncology TI - AXL upregulates c‑Myc expression through AKT and ERK signaling pathways in breast cancers UR - https://doi.org/10.3892/mco.2023.2618 VL - 18 ER -