TY - JOUR AB - The reported effects of nicotine on dendritic cells (DCs) are controversial. To investigate the factors which determine the effects of nicotine on DCs, immature dendritic cells (imDCs) induced from murine bone marrow were treated with different doses of nicotine with or without lipopolysaccharides (LPS). The morphology and expression of the co-stimulatory molecules CD80, CD86, CD40 and CD54 were observed and determined by microscopy and flow cytometry, respectively. The results showed that, firstly, nicotine treatment promoted the development of DC precursors into imDCs with a semi-mature phenotype revealed by a higher expression of CD11c and more branched projections. Secondly, lower doses of nicotine (16.5 ng/ml), but not higher (200 µg/ml), up-regulated the expression of the co-stimulatory molecules CD80, CD40 and CD54 on imDCs. Co-administration of LPS and nicotine revealed differential effects on co-stimulatory molecule expression on imDCs. Thirdly and importantly, treatment with lower doses of nicotine (16.5 ng/ml) did not augment expression of the CD80, CD86, CD40 and CD54 molecules in mature DCs. Fourthly and interestingly, high doses of nicotine (more than 165 µg/ml) revealed pro-apoptotic activity but lower doses of nicotine (16.5-0.165 ng/ml) achieved an anti-apoptotic effect on imDCs. All data presented here indicate that the controversial effects of nicotine on DCs may be due to the LPS of the nicotinic environment and the dose of nicotine used. AD - Department of Respiratory Medicine, The First Affiliated Hospital of Xiamen University, Xiamen 361003, P.R. China Department of Immunology, Basic Medicine Science, Medical College, Xiamen University, Xiamen 361005, P.R. China AU - Hu,Su Xian AU - Sui,Hua Xiu AU - Jin,Hao Jie AU - Ni,Xiao Yan AU - Liu,Xiao Xing AU - Xue,Mao Qiang AU - Zhang,Ye AU - Gao,Feng Guang DA - 2012/04/01 DO - 10.3892/mmr.2012.751 EP - 1010 IS - 4 JO - Mol Med Rep KW - nicotine dendritic cells lipopolysaccharides co-stimulatory molecules PY - 2012 SN - 1791-2997 1791-3004 SP - 1005 ST - Lipopolysaccharide and dose of nicotine determine the effects of nicotine on murine bone marrow-derived dendritic cells T2 - Molecular Medicine Reports TI - Lipopolysaccharide and dose of nicotine determine the effects of nicotine on murine bone marrow-derived dendritic cells UR - https://doi.org/10.3892/mmr.2012.751 VL - 5 ER -