TY - JOUR AB - Survivin is a member of the inhibitor of apoptosis family, which has been suggested to be crucial in the control of cell division and inhibition of apoptosis. Expression of this protein has been observed in transformed cell lines and human tumor tissues, including those from colorectal cancer, but not in terminally differentiated adult tissues. Survivin mRNA expression has frequently been detected in hepatocellular carcinoma (HCC) and its protein expression has been demonstrated to be highly correlated with proliferation index rather than apoptotic index. The present study aimed to analyze the effect of survivin on the tumorigenicity and chemosensitivity of HCC via the establishment of an HCC cell line (PLC/PRF/5) with the stable knockdown of the survivin gene (PLC‑k3). This cell line displayed significantly lower rates of survival and proliferation in assays of cell viability and proliferation, respectively, compared with those of the control cell line (PLC‑v). In addition, PLC‑k3 cells were more sensitive to cisplatin treatment, resulting in S phase arrest. These findings were further confirmed by an in vivo experiment. The data of the present study suggest that survivin is critical in promoting cell proliferation but not in inhibition of apoptosis, and enhances the chemosensitivity of HCC. Thus, the suppression of survivin expression in combination with cisplatin may contribute to the development of more effective treatments for HCC. AD - Department of Surgery, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, SAR, P.R. China AU - Or,Yvonne ,Y.Y. AU - Chow,Ariel ,K.M. AU - Ng,Lui AU - Fan,Sheung,Tat AU - Yau,Thomas ,C.C. AU - Poon,Ronnie ,T.P. AU - Pang,Roberta ,W.C. DA - 2014/10/01 DO - 10.3892/mmr.2014.2413 EP - 2030 IS - 4 JO - Mol Med Rep KW - chemosensitivity cisplatin hepatocellular carcinoma survivin tumorigenesis PY - 2014 SN - 1791-2997 1791-3004 SP - 2025 ST - Survivin depletion inhibits tumor growth and enhances chemosensitivity in hepatocellular carcinoma T2 - Molecular Medicine Reports TI - Survivin depletion inhibits tumor growth and enhances chemosensitivity in hepatocellular carcinoma UR - https://doi.org/10.3892/mmr.2014.2413 VL - 10 ER -