TY - JOUR AB - Ultraviolet (UV) radiation impairs intracellular functions by directly damaging DNA and by indirectly generating reactive oxygen species (ROS), which induce cell cycle arrest and apoptosis. UV radiation can also alter gene expression profiles, including those of mRNA and microRNA (miRNA). The effects of UV radiation on cellular functions and gene expression have been widely documented in human skin cells such as keratinocytes, melanocytes and dermal fibroblasts, but the effect it has on other types of skin cell such as dermal papilla cells, which are crucial in the induction of hair follicle growth, remains unknown. In the current study, the effect of UV radiation on physiological changes and miRNA‑based expression profiles in normal human dermal papilla cells (nHDPs) was investigated. UVB radiation of ≥50 mJ/cm2 displayed high cytotoxicity and apoptosis in a dose‑dependent manner. In addition, ROS generation was exhibited in UVB‑irradiated nHDPs. Furthermore, using miRNA microarray analysis, it was demonstrated that the expression profiles of 42 miRNAs in UVB‑irradiated nHDPs were significantly altered compared with those in the controls (35 upregulated and 7 downregulated). The biological functions of the differentially expressed miRNAs were studied with gene ontology analysis to identify their putative target mRNAs, and were demonstrated to be involved in cell survival‑ and death‑related functions. Overall, the results of the present study provide evidence that miRNA‑based cellular mechanisms may be involved in the UVB‑induced cellular response in nHDPs. AD - Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143‑701, Republic of Korea Coreana Cosmetics Co., Ltd., Cheonan‑si, Chungcheongnam‑do 330‑833, Republic of Korea Laboratory of Molecular Oncology, Cheil General Hospital and Women's Healthcare Center, Kwandong University, College of Medicine, Seoul 100‑380, Republic of Korea Laboratory of Functional Genomics, Korea Institute of Radiological and Medical Sciences, Seoul 139‑706, Republic of Korea Department of Chemistry, Hanyang University, Seoul 133‑791, Republic of Korea Department of Dermatology, Konkuk University School of Medicine, Seoul 143‑701, Republic of Korea Molecular‑Targeted Drug Research Center, Konkuk University, Seoul 143‑701, Republic of Korea AU - Cha,Hwa ,Jun AU - Kim,Ok‑Yeon AU - Lee,Gang ,Tai AU - Lee,Kwang ,Sik AU - Lee,Jae ,Ho AU - Park,In‑Chul AU - Lee,Su‑Jae AU - Kim,Yu ,Ri AU - Ahn,Kyu ,Joong AU - An,In‑Sook AU - An,Sungkwan AU - Bae,Seunghee DA - 2014/10/01 DO - 10.3892/mmr.2014.2418 EP - 1670 IS - 4 JO - Mol Med Rep KW - microRNA ultraviolet B human dermal papilla cells microarray PY - 2014 SN - 1791-2997 1791-3004 SP - 1663 ST - Identification of ultraviolet B radiation‑induced microRNAs in normal human dermal papilla cells T2 - Molecular Medicine Reports TI - Identification of ultraviolet B radiation‑induced microRNAs in normal human dermal papilla cells UR - https://doi.org/10.3892/mmr.2014.2418 VL - 10 ER -