TY - JOUR AB - Cisplatin is an effective anti‑cancer drug; however, one of its side effects is irreversible sensorineural hearing damage. Korean Red Ginseng (KRG) has been used clinically for the treatment of various diseases; however, the underlying mechanism of KRG treatment of ototoxicity has not been studied extensively. The present study aimed to further investigate the mechanism of KRG on cisplatin‑induced toxicity in auditory HEI‑OC1 cells in vitro, as well as in vivo. The pharmacological effects of KRG on cisplatin‑induced changes in the hearing threshold of mice were determined, as well as the effect on the impairment of hair cell arrays. In addition, in order to elucidate the protective mechanisms of KRG, the regulatory effects of KRG on cisplatin‑induced apoptosis‑associated gene levels and nuclear factor‑κB (NF‑κB) activation were investigated in auditory cells. The results revealed that KRG prevented cisplatin‑induced alterations in the hearing threshold of mice as well as the destruction of hair cell arrays in rat organ of Corti primary explants. In addition, KRG inhibited cisplatin‑mediated cell toxicity, reactive oxygen species generation, interleukin‑6 production, cytochrome c release and activation of caspases‑3 in the HEI‑OC1 auditory cell line. Furthermore, the results demonstrated that KRG inhibited the activation of NF‑κB and caspase‑1. In conclusion, these results provided a model for the pharmacological mechanism of KRG and provided evidence for potential therapies against ototoxicity. AD - Department of Cosmeceutical Science, Daegu Hanny University, Kyungsan 712‑715, Republic of Korea Department of Pharmacology, College of Korean Medicine, Institute of Korean Medicine, Kyung Hee University, Seoul 130‑701, Republic of Korea Department of Oriental Pharmacy, College of Pharmacy, Wonkwang‑Oriental Medicines Research Institute, Wonkwang University, Iskan, Jeonbuk 570‑749, Republic of Korea AU - Kim,Su‑Jin AU - Kwak,Hyun ,Jeong AU - Kim,Dae‑Seung AU - Choi,Hyun‑Myung AU - Sim,Jung‑Eun AU - Kim,Sung‑Hoon AU - Um,Jae‑Young AU - Hong,Seung‑Heon DA - 2015/07/01 DO - 10.3892/mmr.2015.3396 EP - 322 IS - 1 JO - Mol Med Rep KW - Korean Red Ginseng cisplatin auditory cell nuclear factor-κB caspase-1 PY - 2015 SN - 1791-2997 1791-3004 SP - 315 ST - Protective mechanism of Korean Red Ginseng in cisplatin-induced ototoxicity through attenuation of nuclear factor-κB and caspase-1 activation T2 - Molecular Medicine Reports TI - Protective mechanism of Korean Red Ginseng in cisplatin-induced ototoxicity through attenuation of nuclear factor-κB and caspase-1 activation UR - https://doi.org/10.3892/mmr.2015.3396 VL - 12 ER -