TY - JOUR AB - Dermal papilla (DP) cells function as important regulators of the hair growth cycle. The loss of these cells is a primary cause of diseases characterized by hair loss, including alopecia, and evidence has revealed significantly increased levels of reactive oxygen species (ROS) in hair tissue and DP cells in the balding population. In the present study, troxerutin, a flavonoid derivative of rutin, was demonstrated to have a protective effect against H2O2‑mediated cellular damage in human DP (HDP) cells. Biochemical assays revealed that pretreatment with troxerutin exerted a protective effect against H2O2‑induced loss of cell viability and H2O2‑induced cell death. Further experiments confirmed that troxerutin inhibited the H2O2‑induced production of ROS and upregulation of senescence‑associated β‑galactosidase activity. Using microRNA (miRNA) microarrays, the present study identified 24 miRNAs, which were differentially expressed in the troxerutin‑pretreated, H2O2‑treated HDP cells. Subsequent prediction using bioinformatics analysis revealed that the altered miRNAs were functionally involved in several cell signaling pathways, including the mitogen‑activated protein kinase and WNT pathways. Overall, these results indicated that ROS‑mediated cellular damage was inhibited by troxerutin and suggested that the use of troxerutin may be an effective approach in the treatment of alopecia. AD - Molecular‑Targeted Drug Research Center and Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143‑701, Republic of Korea Coreana Cosmetics Co., Ltd., Cheonan, Chungcheong 330‑882, Republic of Korea AU - Lim,Kyung,Mi AU - An,Sungkwan AU - Lee,Ok‑Kyu AU - Lee,Myung,Joo AU - Lee,Jeong,Pyo AU - Lee,Kwang,Sik AU - Lee,Ghang,Tai AU - Lee,Kun,Kook AU - Bae,Seunghee DA - 2015/08/01 DO - 10.3892/mmr.2015.3717 EP - 2660 IS - 2 JO - Mol Med Rep KW - microRNA troxerutin antioxidant effect dermal papilla cells senescence PY - 2015 SN - 1791-2997 1791-3004 SP - 2650 ST - Analysis of changes in microRNA expression profiles in response to the troxerutin-mediated antioxidant effect in human dermal papilla cells T2 - Molecular Medicine Reports TI - Analysis of changes in microRNA expression profiles in response to the troxerutin-mediated antioxidant effect in human dermal papilla cells UR - https://doi.org/10.3892/mmr.2015.3717 VL - 12 ER -