TY - JOUR AB - Integrin β1 and β3 expression by podocytes is required to maintain glomerular structural integrity. Previous studies have shown that aldosterone (ALD) is involved in glomerular podocyte injury, and mineralocorticoid receptor (MR) blocker spironolactone effectively reduces proteinuria in patients with diabetic nephropathy. The present study was designed to observe the effects of spironolactone on β1 and β3 integrin expression and podocyte motility under in vitro diabetic conditions. Immortalized mouse podocytes were cultured in media containing normal glucose (NG) levels, high glucose (HG) or HG plus spironolacton. The expression of β1 and β3 integrin in podocytes was detected by reverse transcription quantitative polymerase chain reaction, immunofluorescence and western blot analyses. The effects of spironolacton on podocyte motility was further evaluated using a wound healing assay. HG stimulation markedly decreased mRNA and protein expression of integrin β1, and significantly increased mRNA and protein expression of integrin β3 in cultured podocytes. However, simultaneous treatment with spironolacton (10‑7 mol/l) significantly attenuated HG-mediated increases in integrin β3 and decreases in integrin β1 expression. Furthermore, the migration of podocytes induced by HG was abrogated by concomitant treatment with spironolacton. In conclusion, the present study suggested that HG decreased the expression of integrin β1 in cultured podocytes, accompanied with an increase of integrin β3. Spironolactone inhibited cell motility and stabilized podoctyes treated with HG, probably through partly normalizing the expression of integrin β1 and decreasing the expression of integrin β3. AD - Graduate School, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China Department of Nephrology, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510080, P.R. China AU - Li,Zhuo AU - Zhang,Li AU - Shi,Wei AU - Chen,Yuanhan AU - Zhang,Hong AU - Liu,Shuangxin AU - Liang,Xinling AU - Ling,Ting AU - Yu,Chunping AU - Huang,Zhongshun AU - Tan,Xiaofan AU - Zhao,Xinchen AU - Ye,Zhiming AU - Zhang,Bin AU - Wang,Wenjian AU - Li,Ruizhao AU - Ma,Jianchao DA - 2015/11/01 DO - 10.3892/mmr.2015.4295 EP - 6854 IS - 5 JO - Mol Med Rep KW - spironolactone integrin β1 integrin β3 podocyte PY - 2015 SN - 1791-2997 1791-3004 SP - 6849 ST - Spironolactone inhibits podocyte motility via decreasing integrin β1 and increasing integrin β3 in podocytes under high-glucose conditions T2 - Molecular Medicine Reports TI - Spironolactone inhibits podocyte motility via decreasing integrin β1 and increasing integrin β3 in podocytes under high-glucose conditions UR - https://doi.org/10.3892/mmr.2015.4295 VL - 12 ER -