TY - JOUR AB - Mangiferin, which is a C‑glucosylxanthone (1,3,6,7-tetrahydroxyxanthone-C2-β-D-glucoside) purified from plant sources, has recently gained attention due to its various biological activities. The present study aimed to determine the apoptotic effects of mangiferin on A549 human lung adenocarcinoma cells. In vitro studies demonstrated that mangiferin exerted growth‑inhibitory and apoptosis-inducing effects against A549 cells. In addition, mangiferin exhibited anti-tumor properties in A549 xenograft mice in vivo. Mangiferin triggered G2/M phase cell cycle arrest via downregulating the cyclin-dependent kinase 1-cyclin B1 signaling pathway, and induced apoptotic cell death by inhibiting the protein kinase C-nuclear factor-κB pathway. In addition, mangiferin was able to enhance the antiproliferative effects of cisplatin on A549 cells, thus indicating the potential for a combined therapy. Notably, mangiferin exerted anticancer effects in vivo, where it was able to markedly decrease the volume and weight of subcutaneous tumor mass, and expand the lifespan of xenograft mice. The present study clarified the molecular mechanisms underlying mangiferin-induced antitumor activities, and suggested that mangiferin may be considered a potential antineoplastic drug for the future treatment of cancer. AD - Department of Pediatrics, Sichuan Academy of Medical Science and Sichuan Provincial People's Hospital, Chengdu, Sichuan 610072, P.R. China Guangxi Province Key Laboratory, Guangxi University of Chinese Medicine, Nanning, Guangxi 530200, P.R. China Department of Pharmacy, Sichuan Academy of Medical Science and Sichuan Provincial People's Hospital, Chengdu, Sichuan 610072, P.R. China Institute of Organ Transplantation, Sichuan Academy of Medical Science and Sichuan Provincial People's Hospital, Chengdu, Sichuan 610072, P.R. China Sichuan Academy of Medical Science and Sichuan Provincial People's Hospital, Chengdu, Sichuan 610072, P.R. China AU - Shi,Wei AU - Deng,Jiagang AU - Tong,Rongsheng AU - Yang,Yong AU - He,Xia AU - Lv,Jianzhen AU - Wang,Hailian AU - Deng,Shaoping AU - Qi,Ping AU - Zhang,Dingding AU - Wang,Yi DA - 2016/04/01 DO - 10.3892/mmr.2016.4947 EP - 3432 IS - 4 JO - Mol Med Rep KW - mangiferin cdc2‑cyclin B1 signaling pathway PKC‑NF‑κB pathway PY - 2016 SN - 1791-2997 1791-3004 SP - 3423 ST - Molecular mechanisms underlying mangiferin-induced apoptosis and cell cycle arrest in A549 human lung carcinoma cells T2 - Molecular Medicine Reports TI - Molecular mechanisms underlying mangiferin-induced apoptosis and cell cycle arrest in A549 human lung carcinoma cells UR - https://doi.org/10.3892/mmr.2016.4947 VL - 13 ER -