TY - JOUR AB - A novel T cell-secreted cytokine, termed secreted osteoclastogenic factor of activated T cells (SOFAT) that induces osteoclastic bone resorption in a RANKL-independent manner, has been described. Our group have previously reported that SOFAT is highly expressed in gingival tissues of patients with chronic periodontitis suggesting a putative role in the bone loss associated with periodontal disease. The aim of the present study was to identify other potential cellular sources of SOFAT in the bone resorptive lesions of patients with periodontal disease. Gingival tissues were biopsied from systemically healthy subjects without periodontal disease (n=5) and patients with chronic periodontitis (n=5), and the presence of SOFAT was analyzed by immunohistochemistry and immunofluorescence staining. The present data demonstrated marked SOFAT staining in diseased periodontal tissues that was predominantly associated with the lymphocytic infiltration of gingival tissues. Notably, in addition to CD3+ T cells, B‑lineage cells including plasma cells also exhibited strong staining for SOFAT. As SOFAT has not previously been reported in B‑lineage cells, splenic T cells and B cells were further purified from BALB/c mice and activated using CD3/CD28 and lipopolysaccharide, respectively. SOFAT was quantified by reverse transcription‑quantitative polymerase chain reaction and was shown to be significantly expressed (P<0.05) in both activated T cells and B cells compared with unstimulated cells. These data support a putative role of SOFAT in the bone loss associated with chronic periodontal disease. In addition, to the best of our knowledge, this study demonstrates for the first time that in addition to T cells, B-lineage cells may also be a significant source of SOFAT in inflammatory states. AD - Periodontal Medicine Research Group, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo 13045‑755, Brazil Laboratory of Oral Pathology, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo 13045‑755, Brazil Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo, São Paulo 14049-900, Brazil Atlanta Department of Veterans Affairs Medical Center, Decatur, GA 30033, USA Laboratory of Immunology and Molecular Biology, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo 13045‑755, Brazil AU - Jarry,Christian,R. AU - Martinez,Elizabeth,F. AU - Peruzzo,Daiane,C. AU - Carregaro,Vanessa AU - Sacramento,Laís,A. AU - Araújo,Vera,C. AU - Weitzmann,M.,Neale AU - Napimoga,Marcelo,H. DA - 2016/05/01 DO - 10.3892/mmr.2016.5045 EP - 4258 IS - 5 JO - Mol Med Rep KW - secreted osteoclastogenic factor of activated T cells osteoclast inflammation periodontitis B cell T cell PY - 2016 SN - 1791-2997 1791-3004 SP - 4252 ST - Expression of SOFAT by T- and B-lineage cells may contribute to bone loss T2 - Molecular Medicine Reports TI - Expression of SOFAT by T- and B-lineage cells may contribute to bone loss UR - https://doi.org/10.3892/mmr.2016.5045 VL - 13 ER -