TY - JOUR AB - Angiogenesis is an essential process of cancer progression and is regulated by multiple intracellular signaling pathways, including signal transducer and activator of transcription 3 (STAT3) and sonic hedgehog (SHH). Thus, these pathways have become a promising target for anti‑cancer therapeutic strategies. Oleanolic acid (OA) is an active compound present in various herbal medicines, which have been used historically for the clinical treatment of various types of human malignancies, including colorectal cancer (CRC). The present study used a CRC mouse xenograft model and human umbilical vein endothelial cells (HUVECs) to evaluate the effect of OA on tumor angiogenesis and on the activation of the STAT3 and SHH signaling pathways. It was determined that OA treatment significantly inhibited tumor growth and reduced intratumoral microvessel density (MVD) in CRC mice. In addition, OA treatment inhibited the proliferation, migration and tube formation in HUVECs, in a dose and time-dependent manner. Furthermore, OA markedly suppressed the activation of the STAT3 and SHH signaling pathways and inhibited the expression of the pro‑angiogenic vascular endothelial growth factor A and basic fibroblast growth factor, two important target genes of the aforementioned signaling pathways. Therefore it is suggested that inhibition of tumor angiogenesis via the suppression of multiple signaling pathways may be one of the underlying mechanisms by which OA exerts its anti-cancer effect. AD - Department of Disease Prevention and Healthcare, Fujian Provincial Hospital, Fuzhou, Fujian 350001, P.R. China Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China Hangzhou Naval Sanatorium of Nanjing Military Area Command, Hangzhou, Zhejiang 310002, P.R. China AU - Li,Li AU - Lin,Jiumao AU - Sun,Guodong AU - Wei,Lihui AU - Shen,Aling AU - Zhang,Mingyue AU - Peng,Jun DA - 2016/06/01 DO - 10.3892/mmr.2016.5171 EP - 5282 IS - 6 JO - Mol Med Rep KW - oleanolic acid herbal medicine signaling pathway colorectal cancer angiogenesis PY - 2016 SN - 1791-2997 1791-3004 SP - 5276 ST - Oleanolic acid inhibits colorectal cancer angiogenesis in vivo and in vitro via suppression of STAT3 and Hedgehog pathways T2 - Molecular Medicine Reports TI - Oleanolic acid inhibits colorectal cancer angiogenesis in vivo and in vitro via suppression of STAT3 and Hedgehog pathways UR - https://doi.org/10.3892/mmr.2016.5171 VL - 13 ER -