TY - JOUR AB - The migration of circulating mesenchymal stem cells (MSCs) to injured tissue is an important step in tissue regeneration and requires adhesion to the microvascular endothelium. The current study investigated the underlying mechanism of MSC adhesion to endothelial cells during inflammation. In in vitro MSC culture, tumor necrosis factor‑α (TNF‑α) increased the level of vascular cell adhesion molecule‑1 (VCAM‑1) expression in a dose‑dependent manner. The nuclear factor-κB (NF-κB), extracellular signal‑regulated kinase (ERK) and c‑Jun N‑terminal kinase (JNK) signaling pathway inhibitors, pyrrolidine dithiocarbamate (PDTC), U0126 and SP600125, respectively, suppressed VCAM‑1 expression induced by TNF‑α at the mRNA and protein levels (P<0.05). TNF‑α augmented the activation of NF‑κB, ERK and JNK, and promoted MSC adhesion to human umbilical vein endothelial cells; however, the inhibitors of NF‑κB, ERK and JNK did not affect this process in these cells. The results of the current study indicate that adhesion of circulating MSCs to the endothelium is regulated by TNF-α-induced VCAM-1 expression, which is potentially mediated by the NF‑κB, ERK and JNK signaling pathways. AD - First School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China Department of Hematology, General Hospital of Guangzhou Military Command of Chinese PLA, Guangzhou, Guangdong 510010, P.R. China AU - Lu,Zi‑Yuan AU - Chen,Wan‑Cheng AU - Li,Yong‑Hua AU - Li,Li AU - Zhang,Hang AU - Pang,Yan AU - Xiao,Zhi‑Fang AU - Xiao,Hao‑Wen AU - Xiao,Yang DA - 2016/07/01 DO - 10.3892/mmr.2016.5314 EP - 648 IS - 1 JO - Mol Med Rep KW - mesenchymal stem cells nuclear factor‑κB extracellular signal‑regulated kinase c‑Jun N‑terminal kinase tumor necrosis factor‑α vascular cell adhesion molecule‑1 PY - 2016 SN - 1791-2997 1791-3004 SP - 643 ST - TNF-α enhances vascular cell adhesion molecule-1 expression in human bone marrow mesenchymal stem cells via the NF-κB, ERK and JNK signaling pathways T2 - Molecular Medicine Reports TI - TNF-α enhances vascular cell adhesion molecule-1 expression in human bone marrow mesenchymal stem cells via the NF-κB, ERK and JNK signaling pathways UR - https://doi.org/10.3892/mmr.2016.5314 VL - 14 ER -