TY - JOUR AB - Previous studies have demonstrated that oridonin, a tetracyclic diterpenoid compound extracted from Rabdosia rubescens, inhibits proliferation and induces apoptosis in several tumor cell lines. However, the mechanism by which oridonin inhibits the cell cycle remains poorly understood. In the present study, possible mechanisms by which oridonin affects cell cycle progression were explored in A549 lung cancer cells. Flow cytometry analysis indicated that oridonin inhibited the proliferation of A549 cells by inducing G2/M cell cycle arrest in a dose‑dependent manner. Western blot analysis revealed that in oridonin treated cells, phosphorylated (p‑)ATM serine/threonine kinase (S1981), p‑checkpoint kinase 2 (CHK2) (T68), p‑p53, and phosphorylated H2A histone family member X protein levels were visibly increased, indicating that oridonin promoted G2/M arrest in A549 cells through the ATM‑p53‑CHK2 pathway. This data suggests that oridonin promotes G2/M arrest in A549 cells by facilitating ATM activation, which is likely a common mechanism in other tumor cell types when using this drug for cancer treatment. AD - Department of Respiratory Medicine, Clinical College of Anhui Medical University Affiliated Shenzhen Second People's Hospital, Shenzhen, Guangdong 518035, P.R. China Department of Gastrointestinal Surgery, Clinical College of Anhui Medical University Affiliated Shenzhen Second People's Hospital, Shenzhen, Guangdong 518035, P.R. China School of Medicine, Tongji University, Shanghai 200092, P.R. China Department of Thoracic Surgery, Clinical College of Anhui Medical University Affiliated Shenzhen Second People's Hospital, Shenzhen, Guangdong 518035, P.R. China Renal Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA Department of Respiratory Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China AU - Zheng,Mingxing AU - Zhu,Zhibing AU - Zhao,Yongzhao AU - Yao,Da AU - Wu,Maoqing AU - Sun,Gengyun DA - 2017/01/01 DO - 10.3892/mmr.2016.6008 EP - 379 IS - 1 JO - Mol Med Rep KW - oridonin A549 cells cell cycle arrest p53 ATM PY - 2017 SN - 1791-2997 1791-3004 SP - 375 ST - Oridonin promotes G2/M arrest in A549 cells by facilitating ATM activation T2 - Molecular Medicine Reports TI - Oridonin promotes G2/M arrest in A549 cells by facilitating ATM activation UR - https://doi.org/10.3892/mmr.2016.6008 VL - 15 ER -