TY - JOUR AB - Oridonin is a diterpenoid compound isolated from the medicinal herb Rabdosia rubescens, and has shown marked antitumor effects against different types of cancer. However, the definitive systematic molecular mechanism underlying the antitumor activity of oridonin in multiple myeloma remains to be elucidated. In the present study, cell viability and cytotoxicity were examined to determine the appropriate concentration for proteomic investigation. In addition, cell apoptosis was evaluated using flow cytometry and transmission electron microscopy. A proteomic investigation using a two‑dimensional electrophoresis system and mass spectrometry was performed to identify and characterize the global proteome of the apoptosis induced by oridonin. Of the proteins identified, seven were involved in the anticancer effects of oridonin. Regulation of the expression and function of target proteins, stathmin, dihydrofolate reductase and pyruvate dehydrogenase E1β, may be potential, therapeutic strategies to effectively treat multiple myeloma. These findings provide novel information on the molecular mechanisms underlying the anticancer properties of oridonin in multiple myeloma. AD - Department of Hematology, The First Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China Department of Genetics and Molecular Biology, School of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China Institute of Xi'an Blood Bank, Shaanxi Blood Center, Xi'an, Shaanxi 710061, P.R. China AU - Zhao,Jing AU - Zhang,Mei AU - He,Pengcheng AU - Zhao,Junjie AU - Chen,Ying AU - Qi,Jun AU - Wang,Yuan DA - 2017/04/01 DO - 10.3892/mmr.2017.6213 EP - 1815 IS - 4 JO - Mol Med Rep KW - oridonin LP‑1 cell multiple myeloma apoptosis proteomics PY - 2017 SN - 1791-2997 1791-3004 SP - 1807 ST - Proteomic analysis of oridonin-induced apoptosis in multiple myeloma cells T2 - Molecular Medicine Reports TI - Proteomic analysis of oridonin-induced apoptosis in multiple myeloma cells UR - https://doi.org/10.3892/mmr.2017.6213 VL - 15 ER -