TY - JOUR AB - Previous studies have confirmed that exposure to particulate matter with a diameter of ≤2.5 µm (PM2.5) is associated with inflammation. PM2.5 decreases cardiac cell viability and increases apoptosis through overproduction of reactive oxygen species (ROS). In the present study, the role of PM2.5 in ECs was investigated in vitro. Human umbilical vein endothelial cells and human microvascular endothelial cells (ECs) were incubated with PM2.5 (100‑800 µg/ml) to investigate the effects of PM2.5 on EC viability, migration, tube formation and intracellular levels of ROS. Cell viability and cell apoptosis were determined by MTT assay and flow cytometry analysis. Cell migration was assessed using a Boyden chamber assay, and tube formation was determined by matrigel assay. Tumor necrosis factor‑α and interleukin‑8 levels were measured by ELISA, and ROS levels were assessed with 2',7'‑dichlorofluorescin diacetate. The results indicated that PM2.5 decreases EC viability and increases EC apoptosis in a concentration‑dependent manner. PM2.5 also decreased EC tube formation in a dose‑dependent manner. The results also demonstrated that PM2.5 suppresses adhesion to EC extracellular matrix proteins. Furthermore, PM2.5 exposure significantly induced ROS generation, indicative of oxidative stress. Finally, it was demonstrated that PM2.5 decreased angiogenesis in vivo. These results suggested that repeated exposure to PM2.5 induces vascular inflammation. AD - Department of Emergency, Tongji Hospital Affiliated to Tongji University, Shanghai 200333, P.R. China AU - Chen,Shengguang AU - Wu,Xianzheng AU - Hu,Jiawen AU - Dai,Guoxing AU - Rong,Aihong AU - Guo,Gang DA - 2017/09/01 DO - 10.3892/mmr.2017.6877 EP - 2430 IS - 3 JO - Mol Med Rep KW - particulate matter human umbilical vein endothelial cells human microvascular endothelial cells migration angiogenesis PY - 2017 SN - 1791-2997 1791-3004 SP - 2425 ST - PM2.5 exposure decreases viability, migration and angiogenesis in human umbilical vein endothelial cells and human microvascular endothelial cells T2 - Molecular Medicine Reports TI - PM2.5 exposure decreases viability, migration and angiogenesis in human umbilical vein endothelial cells and human microvascular endothelial cells UR - https://doi.org/10.3892/mmr.2017.6877 VL - 16 ER -