TY - JOUR AB - Forkhead box L2 (FOXL2) is a transcription factor, which is involved in blepharophimosis, ptosis, and epicanthus in versus syndrome (BPES), premature ovarian failure (POF), as well as almost all stages of ovarian development and function. FOXL2 has various target genes, which are implicated in numerous processes, including sex determination, cell cycle regulation and apoptosis and stress response regulation in mammals. However, studies regarding the upstream regulation of FOXL2 are limited. In the present study, the promoter of FOXL2 was successfully cloned and registered in Gen Bank, and a dual luciferase reporter (DLR) analysis demonstrated that the luciferase activity was significantly induced by the promoter of FOXL2. Subsequently, bioinformatics analysis indicated that FOXL2 may be regulated by STAT3, and this was confirmed by a DLR analysis and western blotting, using STAT3 inhibitors. Further study using real‑time cellular analysis indicated that the viability of He La cells was markedly suppressed by STAT3 inhibitors. The present study demonstrated novel findings regarding the upstream regulation of FOXL2 expression and provide a new perspective for future studies in the field. AD - Plastic Surgery Institute, Weifang Medical University, Weifang, Shandong 261053, P.R. China Department of Burns, Weifang People's Hospital, Weifang, Shandong 261053, P.R. China AU - Han,Yangyang AU - Wang,Tianxiao AU - Sun,Shudong AU - Zhai,Zhaohui AU - Tang,Shengjian DA - 2017/09/01 DO - 10.3892/mmr.2017.6914 EP - 2862 IS - 3 JO - Mol Med Rep KW - dual‑luciferase reporter forkhead box L2 promoter signal transducer and activator of transcription 3 transcriptional regulation PY - 2017 SN - 1791-2997 1791-3004 SP - 2856 ST - Cloning of the promoter region of a human gene, FOXL2, and its regulation by STAT3 T2 - Molecular Medicine Reports TI - Cloning of the promoter region of a human gene, FOXL2, and its regulation by STAT3 UR - https://doi.org/10.3892/mmr.2017.6914 VL - 16 ER -