TY - JOUR AB - The present study used microarray analysis to screen the plasma expression of microRNAs (miRNAs) in patients with acute Kawasaki disease (KD) and aimed to explore the pathogenesis of KD. Plasma was collected from children with acute KD (n=6) and from healthy control children (n=6). Total RNA was extracted and differential miRNA expression between the two groups was determined. Differentially expressed miRNAs were validated using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) in an independent cohort (n=8). Target genes of the differentially expressed miRNAs were predicted and analyzed for gene ontology term enrichment and Kyoto Encyclopedia of Genes and Genomes pathways. miRNA microarray analysis revealed that seven miRNAs (miRs) were significantly upregulated (hsa‑let‑7b‑5p, hsa‑miR‑223‑3p, hsa‑miR‑4485, hsa‑miR‑4644, hsa‑miR‑4800‑5p, hsa‑miR‑6510‑5p and hsa‑miR‑765) and three were significantly downregulated (hsa‑miR‑33b‑3p, hsa‑miR‑4443 and hsa‑miR‑4515) in acute KD compared with the healthy controls. hsa‑miR‑223‑3p expression levels detected by RT‑qPCR were consistent with the microarray results. A total of 62 target genes of hsa‑miR‑223‑3p were predicted. In total, 10 differentially expressed miRNAs were identified in acute KD, of which hsa‑miR‑223‑3p was verified by RT‑qPCR. AD - Cardiology Department, Children's Hospital of Soochow University, Suzhou, Jiangsu 215025, P.R. China Radiology Department, Fudan University Affiliated Huashan Hospital, Shanghai 200040, P.R. China Cardiovascular Center, Shenzhen Children's Hospital, Shenzhen, Guangdong 518038, P.R. China AU - Chen,Ye AU - Ding,Yue‑Yue AU - Ren,Yan AU - Cao,Lei AU - Xu,Qiu‑Qin AU - Sun,Ling AU - Xu,Ming‑Guo AU - Lv,Hai‑Tao DA - 2018/01/01 DO - 10.3892/mmr.2017.8016 EP - 938 IS - 1 JO - Mol Med Rep KW - Kawasaki disease microRNAs microRNA‑223‑3p microarray target gene PY - 2018 SN - 1791-2997 1791-3004 SP - 932 ST - Identification of differentially expressed microRNAs in acute Kawasaki disease T2 - Molecular Medicine Reports TI - Identification of differentially expressed microRNAs in acute Kawasaki disease UR - https://doi.org/10.3892/mmr.2017.8016 VL - 17 ER -