TY - JOUR AB - The aim of the present study was to investigate the stimulation of osteoinduced mesenchymal stem cells (MSCs) into chondrogenically predifferentiated MSCs and chondrocytes in a mechanical environment. A novel two‑layer microfluidic chip was used to mimic the interstitial flow in the superficial zones of articular cartilage. The morphology, proliferation rate and the expression of collagen I, collagen II and aggrecan of chondrocytes and chondro‑MSCs were investigated. The results revealed that the cells in the bottom layer were influenced by the top layer's osteoinduced MSCs and the bottom layer's shear flow. The expression of collagen I, which may signify the effect of the shear stress on the dedifferentiation change, was weakened by the stimulation of osteoinduced MSCs on the top layer. The expression of collagen II and aggrecan was increased in the fluidic environment by osteoinduced MSCs. These results indicate that osteoinduced MSCs have a significant effect on the phenotype of chondro‑MSCs and chondrocytes in the fluidic microenvironment. The present study described a simple and promising way to rapidly evaluate cell responses to other cells in a fluidic environment, which may help to better promote the utilization of MSCs and chondrocytes in tissue engineering. AD - Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning 116023, P.R. China Department of Orthopaedics, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116000, P.R. China AU - Bao,Xuanwen AU - Li,Zhongyu AU - Liu,Hui AU - Feng,Ke AU - Yin,Fangchao AU - Li,Hongjing AU - Qin,Jianhua DA - 2018/02/01 DO - 10.3892/mmr.2017.8153 EP - 2288 IS - 2 JO - Mol Med Rep KW - microfluidics mesenchymal stem cells shear stress intercellular interactions metabolism PY - 2018 SN - 1791-2997 1791-3004 SP - 2277 ST - Stimulation of chondrocytes and chondroinduced mesenchymal stem cells by osteoinduced mesenchymal stem cells under a fluid flow stimulus on an integrated microfluidic device T2 - Molecular Medicine Reports TI - Stimulation of chondrocytes and chondroinduced mesenchymal stem cells by osteoinduced mesenchymal stem cells under a fluid flow stimulus on an integrated microfluidic device UR - https://doi.org/10.3892/mmr.2017.8153 VL - 17 ER -