TY - JOUR AB - Osteoporosis is a serious public health problem and icariin (ICA) is the active component of the Epimedium sagittatum, a traditional Chinese medicinal herb. The present study aimed to investigate the effects and underlying mechanisms of ICA as a potential therapy for osteoporosis. Calvaria osteoblasts were isolated from newborn rats and treated with ICA. Cell viability, apoptosis, alkaline phosphatase activity and calcium deposition were analyzed. Bioinformatics analyses were performed to identify differentially expressed proteins (DEPs) in response to ICA treatment. Western blot analysis was performed to validate the expression of DEPs. ICA administration promoted osteoblast viability, alkaline phosphatase activity, calcium deposition and inhibited osteoblast apoptosis. Secretome analysis of ICA‑treated cells was performed using two‑dimensional gel electrophoresis and matrix‑assisted laser desorption/ionization time‑of‑flight mass spectrometry. A total of 56 DEPs were identified, including serpin family F member 1 (PEDF), protein disulfide isomerase family A, member 3 (PDIA3), nuclear protein, co‑activator of histone transcription (NPAT), c‑Myc and heat shock protein 70 (HSP70). These proteins were associated with signaling pathways, including Fas and p53. Bioinformatics and western blot analyses confirmed that the expression levels of the six DEPs were upregulated following ICA treatment. These genes may be directly or indirectly involved in ICA‑mediated osteogenic differentiation and osteogenesis. It was demonstrated that ICA treatment promoted osteogenesis by modulating the expression of PEDF, PDIA3, NPAT and HSP70 through signaling pathways, including Fas and p53. AD - Department of Orthopedics, The 3rd Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210001, P.R. China Reproductive Center, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210023, P.R. China Laboratory Center, The 3rd Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, P.R. China Cardiovascular Department, The 3rd Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, P.R. China School of Medicine and Life Sciences, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, P.R. China AU - Qian,Weiqing AU - Su,Yan AU - Zhang,Yajie AU - Yao,Nianwei AU - Gu,Nin AU - Zhang,Xu AU - Yin,Hong DA - 2018/05/01 DO - 10.3892/mmr.2018.8715 EP - 6525 IS - 5 JO - Mol Med Rep KW - icariin osteoblasts secretome two‑dimensional electrophoresis western blot PY - 2018 SN - 1791-2997 1791-3004 SP - 6515 ST - Secretome analysis of rat osteoblasts during icariin treatment induced osteogenesis T2 - Molecular Medicine Reports TI - Secretome analysis of rat osteoblasts during icariin treatment induced osteogenesis UR - https://doi.org/10.3892/mmr.2018.8715 VL - 17 ER -