TY - JOUR AB - Potassium-chloride cotransporter 2 (KCC2) has been indicated to serve a crucial role during chronic neuropathic pain (NP). Following the emergence of NP, γ‑aminobutyric acid (GABA) A receptor‑mediated signaling may be further impaired by the changes of KCC2 chloride anion gradient. In the present study, the authors investigate the effect of electro-acupuncture (EA) on the behavior and the expression of KCC2 and GABAA receptor γ2 subunit in the spinal cord of chronic constriction injury (CCI) model rats. A total of 60 adult male Sprague‑Dawley rats were divided into four groups: Normal group, sham‑CCI group, CCI group and CCI+EA group. The effect of EA was assessed via the values of mechanical withdrawal threshold and thermal withdrawal latency, which were significantly improved upon stimulation of the ST‑36 and GB‑34 acupoints. In addition, a marked reduction in both the mRNA and protein levels of KCC2 and GABAA receptor γ2 subunit was observed in the spinal cord following loose ligation of the sciatic nerve. The reductions in KCC2 and GABAA receptor γ2 subunit expression were reversed by EA treatment. These results support the notion that KCC2 and GABAA receptor γ2 subunit contribute to NP following peripheral nerve injury and extend the understanding of the analgesic effects of EA on NP. AD - Department of Physical Medicine and Rehabilitation, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China Integrative and Optimized Medicine Research Center, China‑US Institute for Acupuncture and Rehabilitation of Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China Protein Quality Control and Diseases Laboratory, Attardi Institute of Mitochondrial Biomedicine, School of Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China AU - Li,Si‑Si AU - Tu,Wen‑Zhan AU - Jia,Cheng‑Qian AU - Jiang,Xia AU - Qian,Xin‑Ru AU - Yang,Guan‑Hu AU - Hu,Qi‑Miao AU - Chen,Wen‑Ci AU - Lu,Bin AU - Jiang,Song‑He DA - 2018/05/01 DO - 10.3892/mmr.2018.8766 EP - 6968 IS - 5 JO - Mol Med Rep KW - electro‑acupuncture KCC2 GABAA receptor chronic constriction injury analgesic PY - 2018 SN - 1791-2997 1791-3004 SP - 6961 ST - KCC2-GABAA pathway correlates with the analgesic effect of electro-acupuncture in CCI rats T2 - Molecular Medicine Reports TI - KCC2-GABAA pathway correlates with the analgesic effect of electro-acupuncture in CCI rats UR - https://doi.org/10.3892/mmr.2018.8766 VL - 17 ER -