TY - JOUR AB - Liver fibrosis occurs as a result of chronic liver lesions, which may subsequently develop into liver cirrhosis and hepatocellular carcinoma. The involvement of long noncoding RNAs (lncRNAs) in liver fibrosis is being increasingly recognized. However, the exact mechanisms and functions of the majority of lncRNAs are poorly characterized. In the present study, the hepatotoxic substance carbon tetrachloride (CCl4) was employed to induce liver fibrosis in an animal model and agenome‑wide identification of lncRNAs in fibrotic liver tissues compared with CCl4 untreated liver tissues was performed using RNA sequencing. Sprague‑Dawley rats were treated with CCl4 for 8 weeks. Histopathogical alterations were observed in liver tissues, and serum levels of alanine aminotransferase, aspartate aminotransferase, transforming growth factor‑β1 and tumor necrosis factor‑α were significantly higher, in the CCl4‑treated group compared with the CCl4 untreated group. RNA sequencing of liver tissues demonstrated that 231 lncRNAs and 1,036 mRNAs were differentially expressed between the two groups. Furthermore, bioinformatics analysis demonstrated that the differentially expressed mRNAs were predominantly enriched in ʻECM‑receptor interactionʼ, ʻPI3K‑Akt signaling pathwayʼ and ʻfocal adhesionʼ pathways, all of which are essential for liver fibrosis development. Validation of 12 significantly aberrant lncRNAs by reverse transcription‑quantitative polymerase chain reaction indicated that the expression patterns of 11 lncRNAs were consistent with the sequencing data. Furthermore, overexpression of lncRNA NR_002155.1, which was markedly downregulated in CCl4‑treated liver tissues, was demonstrated to inhibit HSC‑T6 cell proliferation in vitro. In conclusion, the present study determined the expression patterns of mRNAs and lncRNAs in fibrotic liver tissue induced by CCl4. The identified differentially expressed lncRNAs may serve as novel diagnostic biomarkers and therapeutic targets for liver fibrosis. AD - Department of Ultrasound, Peking University Shenzhen Hospital, Shenzhen PKU‑HKUST Medical Center, Shenzhen, Guangdong 518036, P.R. China Department of Public Health, Center for Disease Control and Prevention in Jiangxi, Nanchang, Jiangxi 330029, P.R. China Department of Infectious Diseases, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China Department of Digestive Diseases, The Eighth Affiliated Hospital of Sun Yat‑sen University, Shenzhen, Guangdong 518033, P.R. China Department of Infectious Diseases, Peking University Shenzhen Hospital, Shenzhen PKU‑HKUST Medical Center, Shenzhen, Guangdong 518036, P.R. China Department of Traditional Chinese Medicine, Peking University Shenzhen Hospital, Shenzhen PKU‑HKUST Medical Center, Shenzhen, Guangdong 518036, P.R. China AU - Gong,Zhenghua AU - Tang,Jialin AU - Xiang,Tianxin AU - Lin,Jiayu AU - Deng,Chaowen AU - Peng,Yanzhong AU - Zheng,Jie AU - Hu,Guoxin DA - 2018/07/01 DO - 10.3892/mmr.2018.8986 EP - 307 IS - 1 JO - Mol Med Rep KW - long noncoding RNA RNA sequencing liver fibrosis carbon tetrachloride bioinformatics analysis PY - 2018 SN - 1791-2997 1791-3004 SP - 299 ST - Genome‑wide identification of long noncoding RNAs in CCl4‑induced liver fibrosis via RNA sequencing T2 - Molecular Medicine Reports TI - Genome‑wide identification of long noncoding RNAs in CCl4‑induced liver fibrosis via RNA sequencing UR - https://doi.org/10.3892/mmr.2018.8986 VL - 18 ER -