TY - JOUR AB - Liver inflammation and macrophage infiltration are critical steps in the progression of non‑alcoholic fatty liver to the development of non‑alcoholic steatohepatitis. Bone morphogenetic protein‑9 is a cytokine involved in the regulation of chemokines and lipogenesis. However, the function of bone morphogenetic protein‑9 in non‑alcoholic steatohepatitis is still unknown. The present study hypothesized that bone morphogenetic protein‑9 may contribute to steatohepatitis in mice fed a methionine choline deficiency diet (MCD). C57BL/6 mice overexpressing bone morphogenetic protein‑9 and control mice were fed the MCD diet for 4 weeks. Liver tissue and serum samples were obtained for subsequent measurements. Bone morphogenetic protein‑9 overexpression exacerbated steatohepatitis in mice on the MCD diet, as indicated by liver histopathology, increased serum alanine aminotransferase activity, aspartate transaminase activity, hepatic inflammatory gene expression and M1 macrophage recruitment. Although bone morphogenetic protein‑9 overexpression did not affect the expression of pro‑fibrogenic genes, including Collagen I (α)1 or matrix metalloproteinase (MMP) 9, it did upregulate the expression of transforming growth factor‑β and plasminogen activator inhibitor 1, and downregulated the expression of MMP2. The above results indicate that bone morphogenetic protein‑9 exerts a pro‑inflammatory role in MCD diet‑induced non‑alcoholic steatohepatitis. AD - Department of Hepatology and Gastroenterology, Beijing You An Hospital, Capital Medical University, Beijing 100069, P.R. China Division of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China Department of Medicine II, Medical Faculty Mannheim, Heidelberg University, D‑68167 Mannheim, Germany AU - Li,Qi AU - Liu,Beibei AU - Breitkopf‑Heinlein,Katja AU - Weng,Honglei AU - Jiang,Qianqian AU - Dong,Peiling AU - Dooley,Steven AU - Xu,Keshu AU - Ding,Huiguo DA - 2019/09/01 DO - 10.3892/mmr.2019.10508 EP - 2753 IS - 3 JO - Mol Med Rep KW - NASH inflammation macrophage BMP‑9 fibrosis PY - 2019 SN - 1791-2997 1791-3004 SP - 2743 ST - Adenovirus‑mediated overexpression of bone morphogenetic protein‑9 promotes methionine choline deficiency‑induced non‑alcoholic steatohepatitis in non‑obese mice T2 - Molecular Medicine Reports TI - Adenovirus‑mediated overexpression of bone morphogenetic protein‑9 promotes methionine choline deficiency‑induced non‑alcoholic steatohepatitis in non‑obese mice UR - https://doi.org/10.3892/mmr.2019.10508 VL - 20 ER -