TY - JOUR AB - In this study, six compounds were isolated and purified from dandelion, and only sample I exhibited notable antifungal effect on Candida albicans (CA). high‑performance liquid chromatography‑diode‑array detector‑electrospray ionization‑tandem mass spectrometry analysis showed that sample I comprised 4‑coumaric acid, ferulic acid, quercetin pentoside, 3,5‑di‑O‑caffeoylquinic acid, 4,5‑di‑O‑caffeoylquinic acid, luteolin, and two unknown compounds, at a relative percent composition of 11.45, 3.96, 10.48, 34.24, 3.91, 11.80, 3.65 and 4.21%, respectively. Further antimicrobial experiments showed that the minimum inhibitory concentration of sample I was 32.0 mg/ml, and sample I mainly acts on bacterial growth in the exponential phase of CA growth. Optical density and infrared analyses conclusively suggested that sample I damages the structure of CA cells, particularly the cell wall and cell membrane, resulting in macromolecule leakage of intracellular nucleic acids and cell metabolism disruption. In conclusion, dandelion sample I was reported to increase CA cell membrane permeability by affecting the glycosidic bond in β‑(1‑3)‑D glucan and destroying the cell wall, ultimately leading CA to death. AD - Shaanxi Province Key Laboratory of Bio‑Resources, Shaanxi University of Technology, Hanzhong, Shaanxi 723000, P.R. China State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, Qinghai 810016, P.R. China AU - Liang,Yinku AU - Duan,Hongbo AU - Zhang,Ping AU - Han,Hao AU - Gao,Feixiong AU - Li,Yunxiang AU - Xu,Zhongyang DA - 2020/01/01 DO - 10.3892/mmr.2019.10797 EP - 239 IS - 1 JO - Mol Med Rep KW - dandelion active ingredients Candida albicans bacteriostatic mechanism β‑(1‑3)‑D glucan PY - 2020 SN - 1791-2997 1791-3004 SP - 229 ST - Extraction and isolation of the active ingredients of dandelion and its antifungal activity against Candida albicans T2 - Molecular Medicine Reports TI - Extraction and isolation of the active ingredients of dandelion and its antifungal activity against Candida albicans UR - https://doi.org/10.3892/mmr.2019.10797 VL - 21 ER -