TY - JOUR AB - Increasing evidence suggests that intestinal microbiota have critical function in the pathogenesis of inflammatory bowel disease. This present study investigated the effects of Escherichia coli (E. coli) in mice with dextran sulfate sodium (DSS)‑induced colitis. Furthermore, Toll‑like receptor 4 (TLR4) and nuclear factor‑κB (NF‑κB) gene expression was measured by reverse transcription‑quantitative polymerase chain reaction. In total, two experiments were performed. In the first experiment, four groups were established in BALB/c mice: i) Group A, control (no treatments); ii) group B, DSS‑induced colitis; iii) group C, DSS‑induced colitis bacteria depleted (BD) mice; and iv) group D, E. coli‑treated DSS‑induced colitis BD mice. In the second experiment, there were three groups: i) Group A1, control C57BL/6 mice; ii) group B1, E. coli‑treated DSS‑induced colitis BD C57BL/6 mice; and iii) E. coli‑treated DSS‑induced colitis BD TLR4‑/‑ mice. Clinical outcomes, colon and immune histopathology and tissue myeloperoxidase activity were assessed. Mice with DSS‑induced colitis that were treated with E. coli exhibited enhanced recovery, with significantly improved clinical and histological scores compared with the DSS only group. The mRNA expression of TLR4 and NF‑κB in the E. coli‑treated group was also significantly higher. These effects were abolished in TLR4‑/‑ mice, suggesting that E. coli may have promoted recovery through the TLR4 pathway. The present study indicated that E. coli promoted recovery from DSS‑induced colitis in mice, potentially through activation of the TLR4/NF‑κB signaling pathway. AD - Department of Colorectal Surgery, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, Guangdong 510180, P.R. China Department of Cardiology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510120, P.R. China Department of Colorectal Surgery, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, Guangdong 510180, P.R. China AU - Lu,Jiabao AU - Dong,Boye AU - Chen,Ailan AU - He,Feng AU - Peng,Baifu AU - Wu,Zixin AU - Cao,Jie AU - Li,Wanglin DA - 2019/03/01 DO - 10.3892/mmr.2019.9848 EP - 2028 IS - 3 JO - Mol Med Rep KW - ulcerative colitis dextran sulfate sodium‑induced colitis recovery Escherichia coli Toll‑like receptor 4 nuclear factor‑κB PY - 2019 SN - 1791-2997 1791-3004 SP - 2021 ST - Escherichia coli promotes DSS‑induced murine colitis recovery through activation of the TLR4/NF‑κB signaling pathway T2 - Molecular Medicine Reports TI - Escherichia coli promotes DSS‑induced murine colitis recovery through activation of the TLR4/NF‑κB signaling pathway UR - https://doi.org/10.3892/mmr.2019.9848 VL - 19 ER -