TY - JOUR AB - Repulsive guidance molecule a (RGMa) is a membrane‑associated glycoprotein that regulates axonal guidance and inhibits axon outgrowth. In our previous study, we hypothesized that RGMa may be involved in temporal lobe epilepsy (TLE) via the repulsive guidance molecule a (RGMa)‑focal adhesion kinase (FAK)‑Ras signaling pathway. To investigate the role of RGMa in epilepsy, recombinant RGMa protein and FAK inhibitor 14 was intracerebroventricularly injected into a pentylenetetrazol (PTZ) kindling model and Timm staining, co‑immunoprecipitation and western blotting analyses were subsequently performed. The results of the present study revealed that intracerebroventricular injection of recombinant RGMa protein reduced the phosphorylation of FAK (Tyr397) and intracerebroventricular injection of FAK inhibitor 14 reduced the interaction between FAK and p120GAP, as wells as Ras expression. Recombinant RGMa protein and FAK inhibitor 14 exerted seizure‑suppressant effects; however, recombinant RGMa protein but not FAK inhibitor 14 suppressed mossy fiber sprouting in the PTZ kindling model. Collectively, these results demonstrated that RGMa may be considered as a potential therapeutic agent for epilepsy, and that RGMa may exert the aforementioned biological effects partly via the FAK‑p120GAP‑Ras signaling pathway. AD - Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China AU - Song,Mingyu AU - Tian,Fafa AU - Xia,Huang AU - Xie,Yuanyuan DA - 2019/04/01 DO - 10.3892/mmr.2019.9951 EP - 3262 IS - 4 JO - Mol Med Rep KW - repulsive guidance molecule a focal adhesion kinase tyrosine 397 p120GAP mossy fiber sprouting temporal lobe epilepsy PY - 2019 SN - 1791-2997 1791-3004 SP - 3255 ST - Repulsive guidance molecule a suppresses seizures and mossy fiber sprouting via the FAK‑p120RasGAP‑Ras signaling pathway T2 - Molecular Medicine Reports TI - Repulsive guidance molecule a suppresses seizures and mossy fiber sprouting via the FAK‑p120RasGAP‑Ras signaling pathway UR - https://doi.org/10.3892/mmr.2019.9951 VL - 19 ER -