TY - JOUR AB - Respiratory syncytial virus (RSV) infections are associated with significant morbidity and mortality. Inflammation is mediated by cytokine secretion from RSV‑infected airway epithelial cells. Grape seed proanthocyanidin extract (GSPE) exhibits potent antioxidant capacity, as well as anti‑bacterial, anti‑viral, anti‑carcinogenic, anti‑inflammatory and anti‑allergic actions. However, few studies have explored the anti‑inflammatory effects of GSPE on airway epithelial cells infected with RSV. Airway epithelial A549 cells were pretreated with GSPE and its effects on cytokine production during RSV infection were investigated. A549 cells were infected with RSV, with or without GSPE pretreatment, and cultured for 24, 48 and 72 h. The expression of interleukin (IL)‑1β, IL‑6 and IL‑8, were measured by reverse transcription‑quantitative polymerase chain reaction, ELISA and western blotting. RSV infection induced significant increases in proinflammatory cytokine expression. However, GSPE pretreatment decreased the mRNA and protein expression levels of IL‑1ß, IL‑6 and IL‑8. GSPE regulated the immune response by reducing the RSV‑induced transcription of proinflammatory cytokines in airway epithelial cells, suggesting that GSPE helps to prevent RSV‑induced airway disease. AD - Department of Otorhinolaryngology, Eulji University Hospital, Eulji University School of Medicine, Daejeon 35233, Republic of Korea College of Life Sciences & Biotechnology, Korea University, Seoul 02841, Republic of Korea Department of Otorhinolaryngology‑Head and Neck Surgery, Kyung Hee University School of Medicine, Seoul 02447, Republic of Korea AU - Kim,Su ,Jin AU - Lee,Jin‑Woo AU - Eun,Young‑Gyu AU - Lee,Kun ,Hee AU - Yeo,Seung ,Gun AU - Kim,Sung ,Wan DA - 2019/04/01 DO - 10.3892/mmr.2019.9967 EP - 3336 IS - 4 JO - Mol Med Rep KW - proanthocyanidins cytokines inflammation A549 cells respiratory syncytial virus infection PY - 2019 SN - 1791-2997 1791-3004 SP - 3330 ST - Pretreatment with a grape seed proanthocyanidin extract downregulates proinflammatory cytokine expression in airway epithelial cells infected with respiratory syncytial virus T2 - Molecular Medicine Reports TI - Pretreatment with a grape seed proanthocyanidin extract downregulates proinflammatory cytokine expression in airway epithelial cells infected with respiratory syncytial virus UR - https://doi.org/10.3892/mmr.2019.9967 VL - 19 ER -