TY - JOUR AB - Previous studies have shown that long non‑coding RNAs (lncRNAs) serve important roles in necrotizing enterocolitis (NEC). However, the underlying mechanisms remain largely unknown. In order to examine the potential role of lncRNAs in NEC, the present study investigated lncRNA and mRNA expression profiles in NEC lesions and adjacent intestinal tissues using Next Generation Sequencing. A total of 4,202 differentially expressed lncRNAs (fold‑change >2; P<0.05) and 7,860 differentially expressed mRNAs (fold‑change >2; P<0.05) were identified. Moreover, 5 dysregulated lncRNAs and 5 mRNAs were randomly selected, and further assessed by reverse transcription‑quantitative PCR in vitro. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses demonstrated that the differentially expressed lncRNAs were closely associated with NEC, and were enriched in ‘inflammatory response’, ‘Toll‑like receptor binding’, ‘PPAR signaling pathway’, ‘PI3K‑Akt signaling pathway’, ‘transforming growth factor‑β signaling pathway’ and ‘hypoxia‑inducible factor 1 signaling pathway’. In addition, co‑expression analysis demonstrated that these lncRNAs, including lncRNA ENST00000623580, lncRNA NONHSAT180418.1, lncRNA NONHSAT125636.2 and NONHSAT087855.2, may mediate the pathogenesis and development of NEC via lncRNA‑mRNA network interactions. Therefore, the present study provided a novel insight into the role of lncRNAs in NEC. AD - Department of Neonatology, Wuxi Children's Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu 214023, P.R. China AU - Chen,Wenjuan AU - Yan,Xiangyun AU - Tian,Tian AU - Yan,Ru AU - Wang,Xingyun AU - Yu,Zhangbin AU - Li,Yingzheng AU - Zhang,Le AU - Han,Shuping AU - Chen,Wenjuan AU - Yan,Xiangyun AU - Tian,Tian AU - Yan,Ru AU - Wang,Xingyun AU - Yu,Zhangbin AU - Li,Yingzheng AU - Zhang,Le AU - Han,Shuping AU - Chen,Wenjuan AU - Yan,Xiangyun AU - Tian,Tian AU - Yan,Ru AU - Wang,Xingyun AU - Yu,Zhangbin AU - Li,Yingzheng AU - Zhang,Le AU - Han,Shuping DA - 2020/07/01 DO - 10.3892/mmr.2020.11083 EP - 435 IS - 1 JO - Mol Med Rep KW - necrotizing enterocolitis long non‑coding RNAs co‑expression network pathways PY - 2020 SN - 1791-2997 1791-3004 SP - 423 ST - Integrated analysis of a lncRNA‑mRNA network reveals a potential mechanism underlying necrotizing enterocolitis T2 - Molecular Medicine Reports TI - Integrated analysis of a lncRNA‑mRNA network reveals a potential mechanism underlying necrotizing enterocolitis UR - https://doi.org/10.3892/mmr.2020.11083 VL - 22 ER -