TY - JOUR AB - Circular RNAs (circRNAs) are a class of non‑coding RNAs that exhibit important regulatory roles in various biological processes. However, the role of circRNAs and their potential role in osteoblast differentiation and mineralization is unclear. The aim of the present study was to investigate the expression of mmu_circ_003795 and its effect on collagen type XV α 1 chain (COL15A1). First, it was identified that the expression levels of mmu_circ_003795 and osteopontin (OPN) were upregulated in the induced cells. Silencing of mmu_circ_003795 reduced the gene and protein levels of COL15A1 and OPN, whereas the expression level of mmu‑microRNA (miR)‑1249‑5p was upregulated. In addition, after 7 or 14 days of induction, alkaline phosphatase and Alizarin Red‑S staining were decreased in the mmu_circRNA_003795 inhibitory group compared with the negative control group. In conclusion, mmu_circ_003795 may regulate osteoblast differentiation and mineralization in MC3T3‑E1 and MDPC23 cells via mmu‑miR‑1249‑5p by targeting COL15A1. AD - Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510140, P.R. China Faculty of Arts and Science, Human Biology Program, University of Toronto, Toronto, Ontario M5S 1A1, Canada AU - Wu,Jingwen AU - Ren,Wen AU - Zheng,Zhichao AU - Huang,Zhu AU - Liang,Tingting AU - Li,Fuyao AU - Shi,Zhan AU - Jiang,Qianzhou AU - Yang,Xuechao AU - Guo,Lvhua DA - 2020/09/01 DO - 10.3892/mmr.2020.11264 EP - 1746 IS - 3 JO - Mol Med Rep KW - circRNA osteopontin collagen type XV α 1 chain osteoblast differentiation mineralization PY - 2020 SN - 1791-2997 1791-3004 SP - 1737 ST - Mmu_circ_003795 regulates osteoblast differentiation and mineralization in MC3T3‑E1 and MDPC23 by targeting COL15A1 T2 - Molecular Medicine Reports TI - Mmu_circ_003795 regulates osteoblast differentiation and mineralization in MC3T3‑E1 and MDPC23 by targeting COL15A1 UR - https://doi.org/10.3892/mmr.2020.11264 VL - 22 ER -