TY - JOUR AB - Gastric cancer is one of the most common types of cancer worldwide, with a high incidence and mortality rate. MicroRNAs (miRs) play an important role in tumorigenesis, cell proliferation, migration, apoptosis and metastasis of cancer. The present study aimed to investigate the role and potential mechanism of miR‑204‑5p in gastric cancer. The mRNA expression levels of miR‑204‑5p in gastric cancer were determined by reverse transcription‑quantitative PCR. Cell proliferation was determined using Cell Counting Kit‑8 and colony formation assays. Flow cytometry analysis was performed to detect the cell apoptosis rate. Wound healing and Transwell assays were carried out to determine the cell migration and invasion rates, respectively. A putative binding site of miR‑204‑5p in the 3' untranslated region of human epidermal growth factor receptor 2 (HER‑2) was predicted using a bioinformatics algorithm and confirmed using a dual‑luciferase reporter assay. miR‑204‑5p levels were downregulated in gastric cancer cells. Overexpression of miR‑204‑5p significantly inhibited cell proliferation and decreased cell colony formation. Additionally, miR‑204‑5p decreased the migration and invasion rates of gastric cancer cells. Furthermore, an increased apoptotic rate was detected following overexpression of miR‑204‑5p, along with increased expression levels of Bax and decreased expression levels of Bcl‑2. HER‑2 was a direct target of miR‑204‑5p, and inhibition of HER‑2 acted as a tumor suppressor by inhibiting cell proliferation, migration and invasion, and promoting cell apoptosis, which was reversed by the inhibition of miR‑204‑5p expression. These results suggested that miR‑204‑5p could exert its anti‑tumor function by inhibiting cell proliferation, migration and invasion, and promoting cell apoptosis via regulation of HER‑2, which may be a potential therapeutic target for gastric cancer. AD - Department of Traditional Chinese Medicine, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian 362000, P.R. China Department of Ophthalmology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian 362000, P.R. China Department of Traditional Chinese Medicine, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian 362000, P.R. China Department of Endoscopy Room, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian 362000, P.R. China Department of Pathology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian 362000, P.R. China AU - Yang,Shu AU - Chen,Binni AU - Zhang,Baogen AU - Li,Caiping AU - Qiu,Yuena AU - Yang,Hanhui AU - Huang,Zhongxin DA - 2020/10/01 DO - 10.3892/mmr.2020.11367 EP - 2654 IS - 4 JO - Mol Med Rep KW - microRNA‑204‑5p gastric cancer human epidermal growth factor receptor 2 apoptosis PY - 2020 SN - 1791-2997 1791-3004 SP - 2645 ST - miR‑204‑5p promotes apoptosis and inhibits migration of gastric cancer cells by targeting HER‑2 T2 - Molecular Medicine Reports TI - miR‑204‑5p promotes apoptosis and inhibits migration of gastric cancer cells by targeting HER‑2 UR - https://doi.org/10.3892/mmr.2020.11367 VL - 22 ER -