TY - JOUR AB - Infantile hemangioma (IH) is one of the most common vascular tumors that occurs during childhood, but its pathogenesis is currently not completely understood. Even though lncRNA nuclear paraspeckle assembly transcript 1 (NEAT1) plays vital roles in tumorigenesis of malignant tumors, its roles in IH remain unclear. Therefore, we evaluate the function of lncRNA NEAT1 in IH. Reverse transcription‑­quantitative PCR indicated that IH tissues exhibited high expression levels of NEAT1 and hypoxia‑inducible factor 1α (HIF1α), and low expression levels of the microRNA (miR)‑33a‑5p. Small interfering RNA‑mediated depletion of NEAT1 suppressed hemangioma endothelial cell (HemEC) proliferation, migration and invasion. The data suggested that NEAT1 positively regulated HIF1α expression by sponging miR‑33a‑5p in HemECs. miR‑33a‑5p overexpression or HIF1α silencing also acted to suppress HemEC proliferation, migration and invasion. Furthermore, the results indicated that the NEAT1/miR‑33a‑5p/HIF1α axis regulated the NF‑κB signaling pathway. Collectively, the results revealed that depletion of lncRNA NEAT1 suppressed the tumorigenesis of IH by competitively binding miR‑33a‑5p and thereby stimulating the HIF1α/NF‑κB signaling pathway. AD - Department of Dermatology, Kunming Children's Hospital, Kunming, Yunnan 650228, P.R. China Department of Pathology, Kunming Children's Hospital, Kunming, Yunnan 650228, P.R. China Department of Institute Pediatrics, Kunming Children's Hospital, Kunming, Yunnan 650228, P.R. China AU - Yu,Li AU - Shu,Hong AU - Xing,Lu AU - Lv,Meng‑Xing AU - Li,Li AU - Xie,Yu‑Cheng AU - Zhang,Zhao AU - Zhang,Li AU - Xie,Yu‑Yan DA - 2020/10/01 DO - 10.3892/mmr.2020.11409 EP - 3366 IS - 4 JO - Mol Med Rep KW - nuclear paraspeckle assembly transcript 1 microRNA‑33a‑5p hypoxia‑inducible factor 1α NF‑κB signaling pathway infantile hemangioma PY - 2020 SN - 1791-2997 1791-3004 SP - 3358 ST - Silencing long non‑coding RNA NEAT1 suppresses the tumorigenesis of infantile hemangioma by competitively binding miR‑33a‑5p to stimulate HIF1α/NF‑κB pathway T2 - Molecular Medicine Reports TI - Silencing long non‑coding RNA NEAT1 suppresses the tumorigenesis of infantile hemangioma by competitively binding miR‑33a‑5p to stimulate HIF1α/NF‑κB pathway UR - https://doi.org/10.3892/mmr.2020.11409 VL - 22 ER -