TY - JOUR AB - Following influenza A vaccination, certain individuals exhibit adverse reactions in the nervous system, which causes a problem with the safety of the influenza A vaccine. However, to the best of our knowledge, the underlying mechanism of this is unknown. The present study revealed that a monoclonal antibody (H1‑84mAb) against the H1N1 influenza virus hemagglutinin (HA) protein cross‑reacted with an antigen from brain tissue. Total brain tissue protein was immunoprecipitated with this cross‑reactive antibody, and mass spectrometry revealed that the bound antigens were heterogeneous nuclear ribonucleoprotein (hnRNP) A1 and hnRNPA2/B1. Subsequently, the two proteins were expressed in bacteria and it was demonstrated that H1‑84mAb bound to hnRNPA1 and hnRNPA2/B1. These two proteins were expressed in three segments and the cross‑reactivity of H1‑84mAb with the glycine (Gly)‑rich domains of hnRNPA1 (195aa‑320aa) and hnRNPA2/B1 (202aa‑349aa) was determined using ELISA blocking experiments. It was concluded that the Gly‑rich domains of these two proteins are heterophilic antigens that cross‑react with influenza virus HA. The association between the heterophilic antigen Gly‑rich domains and the safety of influenza A vaccines remains to be investigated. AD - Central Laboratory of Shaanxi Provincial People's Hospital, The Third Affiliated Hospital of Medical School, Xi'an Jiaotong University, Shaanxi Province Research Centre of Cell Immunological Engineering and Technology, Key Laboratory of Microbial Infections and Autoimmune Diseases, Xi'an, Shaanxi 710068, P.R. China Guangshui Traditional Chinese Medicine Hospital of Hubei Province, Guangshui, Hubei 432700, P.R. China School of Public Health and Tropical Medicine, Tulane University, New Orleans, LA 70112, USA Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, P.R. China AU - Guo,Chunyan AU - Sun,Lijun AU - Hao,Shuangping AU - Huang,Xiaoyan AU - Hu,Hanyu AU - Liang,Daoyan AU - Feng,Qing AU - Li,Yan AU - Feng,Yangmeng AU - Xie,Xin AU - Hu,Jun DA - 2020/11/01 DO - 10.3892/mmr.2020.11494 EP - 3975 IS - 5 JO - Mol Med Rep KW - influenza virus cross‑reactivity nervous system disease glycine‑rich domain PY - 2020 SN - 1791-2997 1791-3004 SP - 3969 ST - Monoclonal antibody against H1N1 influenza virus hemagglutinin cross reacts with hnRNPA1 and hnRNPA2/B1 T2 - Molecular Medicine Reports TI - Monoclonal antibody against H1N1 influenza virus hemagglutinin cross reacts with hnRNPA1 and hnRNPA2/B1 UR - https://doi.org/10.3892/mmr.2020.11494 VL - 22 ER -