TY - JOUR AB - The altered expression of glycan antigens has been reported during cervix transformation, demonstrating increased mRNA levels of certain glycogenes. Human papillomavirus (HPV) is the aetiological agent of cervical cancer. High risk HPV E5 is considered an oncogene and has been implicated in cell transformation. E6 and E7 HPV oncoproteins modify the expression of certain glycogenes. The role of the E5 HPV protein in glycogene expression changes has not yet been reported. The aim of the present study was to determine the effects of HPV16 E5 oncoprotein on glycogene expression. For these, a microarray assay was performed using the HaCaT cell line and altered glycogenes were identified. The mRNA levels of certain glycogenes were determined via reverse transcription‑quantitative PCR (RT‑qPCR). Using in silico analysis, the present study identified that glycosylation pathways were altered by E5. Microarray analysis revealed alterations in certain glycogenes, including the upregulation of ST6GAL1, ST3GAL3, CHST2 and MANBA, and the downregulation of UGT2B15, GALNT11, NDST2 and UGT1A10. Increased mRNA levels were confirmed via RT‑qPCR for sialyltransferases genes. Additionally, in silico analysis was performed to identify glycosylation networks altered in the presence of the E5 oncoprotein. The analysis revealed that E5 could modify glycan sialylation, the N‑glycosylation pathway, keratan sulfate and glycosaminoglycan synthesis. To the best of our knowledge, the current study was the first to determine the role of the HPV16 E5 oncoprotein in glycogene expression changes. The results indicated that increased sialyltransferase mRNA levels reported in pre‑malignant and malignant cervical tissues could be the result of E5 oncoprotein expression. The results provide a possible role of HPV infection on glycosylation changes reported during cervix transformation. AD - Laboratory of Molecular Biology, East Biomedical Research Center, Mexican Institute of Social Security, Metepec 74360, Mexico Research Center of Microbiological Sciences, Institute of Sciences, Meritorious Autonomous University of Puebla, Puebla 72592, Mexico Consejo Nacional de Ciencia y Tecnología-Centro de Investigación Biomédica de Oriente, Metepec 74360, Mexico West Biomedical Research Center, Mexican Institute of Social Security, Guadalajara 44290, Mexico AU - Cisneros-Ramírez,Denisse AU - Martínez-Laguna,Ygnacio AU - Martínez-Morales,Patricia AU - Aguilar-Lemarroy,Adriana AU - Jave-Suárez,Luis ,Felipe AU - Santos-López,Gerardo AU - Reyes-Leyva,Julio AU - Vallejo-Ruiz,Verónica DA - 2020/12/01 DO - 10.3892/mmr.2020.11630 EP - 5453 IS - 6 JO - Mol Med Rep KW - human papillomavirus 16 E5 oncoprotein human papillomavirus glycogene glycosylation microarray expression PY - 2020 SN - 1791-2997 1791-3004 SP - 5444 ST - Glycogene expression profiles from a HaCaT cell line stably transfected with HPV16 E5 oncogene T2 - Molecular Medicine Reports TI - Glycogene expression profiles from a HaCaT cell line stably transfected with HPV16 E5 oncogene UR - https://doi.org/10.3892/mmr.2020.11630 VL - 22 ER -