TY - JOUR AB - Chaperone‑mediated autophagy (CMA) is a selective type of autophagy whereby a specific subset of intracellular proteins is targeted to the lysosome for degradation. The present study investigated the mechanisms underlying the response and resistance to 5‑fluorouracil (5‑FU) in colorectal cancer (CRC) cell lines. In engineered 5‑FU‑resistant CRC cell lines, a significant elevation of lysosome‑associated membrane protein 2A (LAMP2A), which is the key molecule in the CMA pathway, was identified. High expression of LAMP2A was found to be responsible for 5‑FU resistance and to enhance PLD2 expression through the activation of NF‑κB pathway. Accordingly, loss or gain of function of LAMP2A in 5‑FU‑resistant CRC cells rendered them sensitive or resistant to 5‑FU, respectively. Taken together, the results of the present study suggested that chemoresistance in patients with CRC may be mediated by enhancing CMA. Thus, CMA is a promising predictor of chemosensitivity to 5‑FU treatment and anti‑CMA therapy may be a novel therapeutic option for patients with CRC. AD - Department of Experimental Oncology, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China AU - Xuan,Ying AU - Zhao,Shuang AU - Xiao,Xingjun AU - Xiang,Liwei AU - Zheng,Hua-Chuan DA - 2021/05/01 DO - 10.3892/mmr.2021.11999 IS - 5 JO - Mol Med Rep KW - chaperone‑mediated autophagy lysosome‑associated membrane protein 2A colorectal cancer drug resistance NF‑κB p65 pathway 5‑fluorouracil PY - 2021 SN - 1791-2997 1791-3004 SP - 360 ST - Inhibition of chaperone‑mediated autophagy reduces tumor growth and metastasis and promotes drug sensitivity in colorectal cancer T2 - Molecular Medicine Reports TI - Inhibition of chaperone‑mediated autophagy reduces tumor growth and metastasis and promotes drug sensitivity in colorectal cancer UR - https://doi.org/10.3892/mmr.2021.11999 VL - 23 ER -