TY - JOUR AB - Glioblastomas (GBMs) are the most frequent and malignant type of brain tumor. It has been reported that progesterone (P4) regulates the progression of GBMs by modifying the expression of genes that promote cell proliferation, migration and invasion; however, it is not fully understood how these processes are regulated. It is possible that P4 mediates some of these effects through changes in the microRNA (miRNA) expression profile in GBM cells. The present study investigated the effects of P4 on miRNAs expression profile in U‑251MG cells derived from a human GBM. U‑251MG cells were treated for 6 h with P4, RU486 (an antagonist of the intracellular progesterone receptor), the combined treatment (P4+RU486) and cyclodextrin (vehicle) and then a miRNA microarray analysis conducted. The expression analysis revealed a set of 190 miRNAs with differential expression in the treatments of P4, RU486 and P4+RU486 in respect to the vehicle and P4 in respect to P4+RU486, of which only 16 were exclusively regulated by P4. The possible mRNA targets of the miRNAs regulated by P4 could participate in the regulation of proliferation, cell cycle progression and cell migration of GBMs. The present study provided insight for understanding epigenetic modifications regulated by sex hormones involved in GBM progression, and for identifying potential therapeutic strategies for these brain tumors. AD - Unidad de Investigación en Reproducción Humana, Instituto Nacional de Perinatología‑Facultad de Química, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico Oncogenomics Laboratory, The National Institute of Genomic Medicine, Mexico City 14610, Mexico Laboratory of Cell Communication and Extracellular Vesicles, The National Institute of Genomic Medicine, Mexico City 14610, Mexico AU - Velázquez‑Vázquez,Diana,Elisa AU - Del Moral‑Morales,Aylin AU - Cruz‑Burgos,Jenie  ,Marian AU - Martínez‑Martínez,Eduardo  AU - Rodríguez‑Dorantes,Mauricio  AU - Camacho‑Αrroyo,Ignacio DA - 2021/06/01 DO - 10.3892/mmr.2021.12114 IS - 6 JO - Mol Med Rep KW - microRNAs glioblastomas microarrays progesterone progesterone receptor brain tumors PY - 2021 SN - 1791-2997 1791-3004 SP - 475 ST - Expression analysis of progesterone‑regulated miRNAs in cells derived from human glioblastoma T2 - Molecular Medicine Reports TI - Expression analysis of progesterone‑regulated miRNAs in cells derived from human glioblastoma UR - https://doi.org/10.3892/mmr.2021.12114 VL - 23 ER -