TY - JOUR AB - As is well known, dexmedetomidine (DEX) serves a neuroprotective role in cerebral ischemia‑reperfusion (CIR) injury, and microRNA (miR)‑199a has been re‑ported to be associated with IR injury. However, the association between DEX and miR‑199a in CIR injury remains unknown. Thus, the aim of the present study was to verify whether the neuroprotective effect of DEX on cerebral ischemia‑reperfusion rats is associated with miR‑199a. A rat model of CIR was established, and the modified neurological severity score (mNSS) was evaluated. The effect of DEX on the patholog‑ical structure of the cerebral cortex in CIR rats was observed by hematoxylin and eosin and Nissl staining. Reverse transcription‑quantitative PCR was used to analyze the expression levels of miR‑199a in brain tissue following intracerebroventricular injection of miR‑199a antagomir. The co‑expression of NeuN and microtubule‑associated proteins 1A/1B light chain 3B in the cerebral cortex was analyzed by immunofluorescence staining. Western blotting and immunohistochemistry were performed to analyze the expression of autophagy‑associated proteins in the brain tissue. DEX inhibited the expression of miR‑199a, decreased the mNSS and improved pathological damage to the cerebral cortex. DEX also inhibited autophagy and expression levels of associated proteins and decreased nerve cell injury. In conclusion, DEX inhibited expression of miR‑199a and improved neurocyte injury induced by CIR. AD - Department of Anesthesiology, Yantaishan Hospital, Yantai, Shandong 264000, P.R. China Department of Anesthesiology, The Second Hospital of Liaocheng Affiliated to Shan‑dong First Medical University, Liaocheng, Shandong 252000, P.R. China Department of Anesthesiology, Laixi People's Hospital, Qingdao, Shandong 266600, P.R. China Department of Anesthesiology, Zibo Maternal and Child Health Hospital, Zibo, Shandong 255000, P.R. China AU - Zhu,Yulin AU - Zhao,Huatang AU - Zhang,Wenshan AU - Ma,Xingang AU - Liu,Ye DA - 2021/08/01 DO - 10.3892/mmr.2021.12213 IS - 2 JO - Mol Med Rep KW - dexmedetomidine cerebral ischemia‑reperfusion microRNA‑199a autophagy PY - 2021 SN - 1791-2997 1791-3004 SP - 574 ST - Dexmedetomidine attenuates neuronal injury induced by cerebral ischemia‑reperfusion by regulating miR‑199a T2 - Molecular Medicine Reports TI - Dexmedetomidine attenuates neuronal injury induced by cerebral ischemia‑reperfusion by regulating miR‑199a UR - https://doi.org/10.3892/mmr.2021.12213 VL - 24 ER -