TY - JOUR AB - Bladder cancer has high morbidity and mortality rates among the male genitourinary system tumor types. MicroRNA‑218 (miR‑218) is associated with the development of a variety of cancer types, including bladder cancer. Rab6c is a member of the Rab family and is involved in drug resistance in MCF7 cells. The aim of the present study was to clarify the relationship between Rab6c and miR‑218 in bladder cancer cell lines. In this study, the expression levels of miR‑218 and Rab6c were evaluated via reverse transcription‑quantitative PCR and western blotting, respectively. The association between Rab6c and miR‑218 was recognized via TargetScan analysis and dual luciferase reporter gene detection. Cell proliferation was analyzed using Cell Counting Kit‑8 and colony formation assays, and the invasive ability was measured via Transwell assays. Rab6c was highly expressed in bladder cancer, while miR‑218 had abnormally low expression in bladder cancer. In addition, there was a mutual regulation between Rab6c and miR‑218 in bladder cancer. It was found that overexpression of Rab6c significantly enhanced the proliferation, colony formation and invasion of T24 and EJ cells. Furthermore, miR‑218 overexpression blocked the promoting effects of Rab6c on the malignant behavior of bladder cancer cells. Thus, Rab6c promotes the proliferation and invasion of bladder cancer cells, while miR‑218 has the opposite effect, which may provide a novel insight for the treatment of bladder cancer. AD - Department of Urology, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, Jiangsu 225003, P.R. China Key Laboratory of Pattern Recognition in Liaoning, School of Medical Devices, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, P.R. China Department of The Second Cadre Ward, General Hospital of Northern Theater Command, National Center for Clinical Research of Geriatric Diseases, Shenyang, Liaoning 157099, P.R. China Department of Urology, General Hospital of Northern Theater Command, Shenyang, Liaoning 110013, P.R. China Department of Oncology, General Hospital of Northern Theater Command, Shenyang, Liaoning 110013, P.R. China AU - He,Long AU - Pan,Xiang AU - Wang,Xialu AU - Cao,Yuhua AU - Chen,Peng AU - Du,Cheng AU - Huang,Daifa DA - 2021/11/01 DO - 10.3892/mmr.2021.12432 IS - 5 JO - Mol Med Rep KW - Rab6c microRNA‑218 bladder cancer proliferation invasion PY - 2021 SN - 1791-2997 1791-3004 SP - 792 ST - Rab6c is a new target of miR‑218 that can promote the progression of bladder cancer T2 - Molecular Medicine Reports TI - Rab6c is a new target of miR‑218 that can promote the progression of bladder cancer UR - https://doi.org/10.3892/mmr.2021.12432 VL - 24 ER -