TY - JOUR AB - Primary nephrotic syndrome (PNS) is the commonest glomerular disease affecting children. Previous studies have confirmed that CXC motif chemokine ligand 16 (CXCL16) is involved in the pathogenesis of PNS. However, the exact mechanisms underlying the pathogenesis of PNS remain to be elucidated. Thus, the present study aimed to elucidate the role of CXCL16 in PNS. It was found that the expression of CXCL16 and extracellular signal‑regulated kinases 1 and 2 (ERK1/2) were significantly increased in clinical PNS renal tissues using reverse transcription‑quantitative PCR, western blot analysis and immunohistochemistry. Lentivirus overexpression or short hairpin RNA vector was used to induce the overexpression or knockdown of CXCL16 in podocytes, respectively. Overexpression of CXCL16 in podocytes could decrease the cell proliferation and increase the migration and apoptosis, whereas CXCL16 knockdown increased cell proliferation and decreased cell migration and apoptosis. Results of the present study further demonstrated that ERK2 protein expression was regulated by CXCL16. The knockdown of ERK2 expression reversed the effects of CXCL16 on the proliferation, apoptosis, migration and epithelial mesenchymal transition (EMT) of podocytes. Collectively, the findings of the present study highlighted that the CXCL16/ERK1/2 pathway regulates the growth, migration, apoptosis and EMT of human podocytes. AD - Department of Pediatrics, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, P.R. China Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, P.R. China Department of Pediatrics, People's Hospital of Rizhao, Rizhao, Shandong 276800, P.R. China AU - Chen,Yuan AU - Wang,Zhiyi AU - Li,Qian AU - Tian,Minle AU - Zhu,Yanji AU - Yu,Lichun AU - Wang,Jing AU - Sun,Shuzhen DA - 2022/06/01 DO - 10.3892/mmr.2022.12728 IS - 6 JO - Mol Med Rep KW - CXC motif chemokine ligand 16 extracellular signal‑regulated kinases 1 and 2 epithelial mesenchymal transition podocytes injury primary nephrotic syndrome PY - 2022 SN - 1791-2997 1791-3004 SP - 212 ST - CXCL16/ERK1/2 pathway regulates human podocytes growth, migration, apoptosis and epithelial mesenchymal transition T2 - Molecular Medicine Reports TI - CXCL16/ERK1/2 pathway regulates human podocytes growth, migration, apoptosis and epithelial mesenchymal transition UR - https://doi.org/10.3892/mmr.2022.12728 VL - 25 ER -