TY - JOUR AB - Hepatocellular carcinoma (HCC) is the leading cause of cancer‑associated death in the world. Chemotherapy remains the primary treatment method for HCC. Despite advances in chemotherapy and modalities, recurrence and resistance limit therapeutic success. Salidroside (Sal), a bioactive component extracted from the rhizome of Rhodiola rosea L, exhibits a spectrum of biological activities including antitumor effects. In the present study, it was demonstrated that Sal could induce apoptosis and autophagy of 97H cells by using CCK‑8 assay, transmission electron microscopy (TEM), Hoechst33342 staining, MDC staining, western blotting. Pretreatment with Sal enhanced apoptosis and autophagy via upregulation of expression levels of Bax, Caspase‑3, Caspase‑9, light chain (LC)3‑II and Beclin‑1 proteins and downregulation of expression levels of Bcl‑2, LC3‑I and p62 protein in 97H cells. Furthermore, Sal was demonstrated to inhibit activation of the PI3K/Akt/mTOR signaling pathway and, when combined with autophagy inhibitor chloroquine diphosphate (CQ), increased phosphorylation of PI3K, Akt and mTOR proteins. The combined treatment with Sal and CQ not only decreased Sal‑induced autophagy, but also accelerated Sal‑induced apoptosis. Therefore, Sal‑induced autophagy might serve a role as a defense mechanism in human liver cancer cells and its inhibition may be a promising strategy for the adjuvant chemotherapy of liver cancer. AD - School of Basic Medicine, Gansu University of Traditional Chinese Medicine, Lanzhou, Gansu 730000, P.R. China Translational Medicine Research Center, Gansu Provincial Academic Institute for Medical Research, Gansu Provincial Cancer Hospital, Lanzhou, Gansu 730050, P.R. China Clinical College of Integrated Chinese and Western Medicine, Guangxi University of Traditional Chinese Medicine, Nanning, Guangxi 530200, P.R. China AU - Jiang,Bing AU - Feng,Longfei AU - Yang,Tao AU - Guo,Wenjing AU - Li,Yangyang AU - Wang,Tao AU - Liu,Chengguang AU - Su,Haixiang DA - 2023/02/01 DO - 10.3892/mmr.2022.12924 IS - 2 JO - Mol Med Rep KW - salidroside chloroquine diphosphate liver cancer apoptosis autophagy PI3K/Akt/mTOR PY - 2023 SN - 1791-2997 1791-3004 SP - 37 ST - Combination of chloroquine diphosphate and salidroside induces human liver cell apoptosis via regulation of mitochondrial dysfunction and autophagy T2 - Molecular Medicine Reports TI - Combination of chloroquine diphosphate and salidroside induces human liver cell apoptosis via regulation of mitochondrial dysfunction and autophagy UR - https://doi.org/10.3892/mmr.2022.12924 VL - 27 ER -