TY - JOUR AB - Sanguisorba officinalis L. has been used in traditional Asian medicine to treat diseases including diarrhea, chronic intestinal infections, duodenal ulcers and bleeding. This study examined the antiproliferative effects and apoptotic activity of hot water extract of S. officinalis L. (HESO) on HSC4 and HN22 human oral cancer cells. The effects of HESO were evaluated by the 3-(4,5-dimethylthiazol-20yl)-(3-carboxymethoxyphenyl)-2-(4-sulphophenyl)-2H-tetrazolium (MTS) assay, 4'-6-diamidino-2-phenylindole (DAPI) staining and western blot analysis. HESO was found to inhibit cell growth and induce apoptosis in HSC4 and HN22 oral cancer cells. HESO downregulated myeloid cell leukemia-1 (Mcl-1) in HSC4 cells and was associated with the activation of Bak, resulting in Bak oligomerization on the mitochondrial outer membrane. HESO did not alter Mcl-1 expression in HN22 cells, but it decreased Sp1 expression. The downregulation of Sp1 by HESO in HN22 cells resulted in a decrease in survivin, a downstream target protein of Sp1. These results suggested that HESO inhibited the growth of oral cancer through either Mcl-1 or Sp1, indicating that HESO may serve as a potential drug candidate against oral cancer. AD - Department of Oral Pathology, School of Dentistry, Institute of Oral Bioscience, Chonbuk National University, Jeonju, Republic of Korea R__AMB__D Center, Biterials Co. Ltd., Seoul, Republic of Korea Department of Food Science and Nutrition, College of Health Welfare and Education, Gwangju University, Gwangju, Republic of Korea Lee Gil Ya Cancer and Diabetes Institute, Gachon University of Medicine and Science, Inchon, Republic of Korea Department of Companion and Laboratory Animal Science, Kongju National University, Yesan, Republic of Korea AU - Shin,Ji-Ae AU - Kim,Jun-Sung AU - Kwon,Ki-Han AU - Nam,Jeong-Seok AU - Jung,Ji‑Youn AU - Cho,Nam-Pyo AU - Cho,Sung-Dae DA - 2012/09/01 DO - 10.3892/ol.2012.748 EP - 494 IS - 3 JO - Oncol Lett KW - Sanguisorba officinalis L. myeloid cell leukemia-1 specificity protein 1 oral cancer apoptosis PY - 2012 SN - 1792-1074 1792-1082 SP - 489 ST - Apoptotic effect of hot water extract of Sanguisorba officinalis L. in human oral cancer cells T2 - Oncology Letters TI - Apoptotic effect of hot water extract of Sanguisorba officinalis L. in human oral cancer cells UR - https://doi.org/10.3892/ol.2012.748 VL - 4 ER -