TY - JOUR AB - Bladder cancer (BC) is universally acknowledged as a significant public health issue, worldwide. Numerous studies have demonstrated that cigarette smoke is the primary risk factor for BC. However, the mechanism of cigarette smoke‑induced BC has not been fully elucidated. Sustained epithelial cell hyperplasia has been identified as a preneoplastic lesion during the formation of BC. The aim of the present study was to investigate whether exposure to cigarette smoke extract (CSE) induced proliferation in normal human urothelial SV‑HUC‑1 cells. Furthermore, the role of the mitogen‑activated protein kinase (MAPK)/activator protein‑1 (AP‑1) pathway in the CSE‑induced proliferation of SV‑HUC‑1 cells was also investigated. The present study revealed that the expression of phosphorylated‑extracellular signal regulated protein kinase (ERK)1/2, Jun N‑terminal kinase (JNK) and p38 was significantly increased following exposure to CSE in SV‑HUC‑1 cells. Furthermore, CSE increased the expression of the proliferation markers, cyclin D1 and proliferating cell nuclear antigen. By contrast, CSE attenuated the expression of p21. In addition, the inhibitors of ERK1/2 and JNK reversed the aforementioned effects of CSE. However, p38 inhibition did not reverse CSE‑induced proliferation. In conclusion, the results of the present study demonstrated that exposure to CSE induced proliferation in normal human urothelial cells. Furthermore, the results also indicated that the ERK1/2 and JNK pathways are important for the regulation of proliferation via the AP‑1 proteins. AD - Department of Urology, The Second Hospital of Anhui Medical University, Hefei, Anhui 230032, P.R. China Department of Nutrition and Food Safety, School of Public Health, Nanjing Medical University, Nanjing, Jiangsu 211166, P.R. China AU - Geng,Hao AU - Zhao,Li AU - Liang,Zhaofeng AU - Zhang,Zhiqiang AU - Xie,Dongdong AU - Bi,Liangkuan AU - Wang,Yi AU - Zhang,Tao AU - Cheng,Lei AU - Yu,Dexin AU - Zhong,Caiyun DA - 2017/01/01 DO - 10.3892/ol.2016.5407 EP - 475 IS - 1 JO - Oncol Lett KW - bladder cancer cigarette smoke extract proliferation mitogen-activated protein kinase/activator protein‑1 pathway PY - 2017 SN - 1792-1074 1792-1082 SP - 469 ST - Cigarette smoke extract-induced proliferation of normal human urothelial cells via the MAPK/AP‑1 pathway T2 - Oncology Letters TI - Cigarette smoke extract-induced proliferation of normal human urothelial cells via the MAPK/AP‑1 pathway UR - https://doi.org/10.3892/ol.2016.5407 VL - 13 ER -