TY - JOUR AB - Although numerous effective therapies have improved the survival rate of patients with breast cancer, a number of patients present with tumor recurrence and metastasis. A liquid biopsy of circulating tumor cells (CTC) is a non‑invasive method to obtain tumor cells and may be used as substitute for a tumor tissue biopsy. The present study focuses on determining whether CTC culture is an optimal method of obtaining sufficient amounts of CTCs for molecular analysis. The current study demonstrates a method of isolating and culturing CTCs from patients with breast cancer and the construction of a molecular profile of cultured cells using the Ion AmpliSeq Cancer Gene Panel V2. Gene mutations that were observed in cultured CTCs were compared with those observed in primary tumor tissues. CTCs were isolated and cultured from the blood of six patients with breast cancer. Mutations from the Catalogue Of Somatic Mutation In Cancer (COSMIC) were detected in Platelet‑Derived Growth Factor Receptor Alpha, MET (also known as Hepatocyte Growth Factor Receptor), Phosphatase and Tensin Homolog, Harvey Rat Sarcoma Viral Oncogene Homolog, SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin Subfamily B Member 1, Cyclin Dependent Kinase Inhibitor 2A and MutL Homolog 1 genes in 5/6 samples. A comparison between mutations detected in cultured CTCs and mutations detected in primary tumor tissues demonstrated that a large number of mutations that were identified in CTCs were also detected in primary tumor tissues. The results from the current study describe a novel cell culture approach that may be used to obtain an optimal number of CTCs for molecular analysis. This novel approach is able to be used as a tool for liquid biopsy during breast cancer treatment. AD - Cytogen Incorporated, Songpa, Seoul 138‑961, Republic of Korea Department of Surgery, Asan Medical Center, College of Medicine, University of Ulsan, Songpa, Seoul 138‑736, Republic of Korea Macrogen Incorporated, Geumcheon, Seoul 153‑781, Republic of Korea AU - Hwang,Eunjoo AU - Uh,Ji‑Hyun AU - Lee,Hye ,Seon AU - Lee,Cham ,Han AU - Lee,Soo ,Jeong AU - Ahn,Sei ,Hyun AU - Son,Byung ,Ho AU - Lee,Jong ,Won AU - Yu,Jong ,Han AU - Kwon,Nak‑Jung AU - Lee,Woo ,Chung AU - Yang,Kap‑Seok AU - Choi,Sung ,Ho AU - Kim,Myoung ,Shin AU - Lee,Jinseon AU - Jeon,Byung ,Hee DA - 2017/06/01 DO - 10.3892/ol.2017.6077 EP - 4632 IS - 6 JO - Oncol Lett KW - breast cancer circulating tumor cells culture cancer gene panel analysis liquid biopsy PY - 2017 SN - 1792-1074 1792-1082 SP - 4627 ST - Cancer gene panel analysis of cultured circulating tumor cells and primary tumor tissue from patients with breast cancer T2 - Oncology Letters TI - Cancer gene panel analysis of cultured circulating tumor cells and primary tumor tissue from patients with breast cancer UR - https://doi.org/10.3892/ol.2017.6077 VL - 13 ER -