TY - JOUR AB - Human gastric adenocarcinoma (AGS) is one of the most common types of malignant tumor and the third‑leading cause of tumor‑associated mortality worldwide. Withaferin A (WA), a steroidal lactone derived from Withania somnifera, exhibits antitumor activity in a variety of cancer models. However, to the best of our knowledge, the direct effect of WA on AGS cells has not previously been determined. The present study investigated the effects of WA on the proliferation and metastatic activity of AGS cells. WA exerted a dose‑dependent cytotoxic effect on AGS cells. The effect was associated with cell cycle arrest at the G2/M phase and the expression of apoptotic proteins. Additionally, WA treatment resulted in a decrease in the migration and invasion ability of the AGS cells, as demonstrated using a wound healing assay and a Boyden chamber assay. These results indicate that WA directly inhibits the proliferation and metastatic activity of gastric cancer cells, and suggest that WA may be developed as a drug for the treatment of gastric cancer. AD - Laboratory Animal Medicine, College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Republic of Korea Department of Microbiology and Immunology, David H. Smith Center for Vaccine Biology and Immunology, University of Rochester, Rochester, NY 14642, USA National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju, Chungcheongbuk 28116, Republic of Korea AU - Kim,Green AU - Kim,Tae‑Hyoun AU - Hwang,Eun‑Ha AU - Chang,Kyu‑Tae AU - Hong,Jung,Joo AU - Park, Jong‑Hwan DA - 2017/07/01 DO - 10.3892/ol.2017.6169 EP - 422 IS - 1 JO - Oncol Lett KW - withaferin A gastric cancer proliferation apoptosis cell cycle arrest migration PY - 2017 SN - 1792-1074 1792-1082 SP - 416 ST - Withaferin A inhibits the proliferation of gastric cancer cells by inducing G2/M cell cycle arrest and apoptosis T2 - Oncology Letters TI - Withaferin A inhibits the proliferation of gastric cancer cells by inducing G2/M cell cycle arrest and apoptosis UR - https://doi.org/10.3892/ol.2017.6169 VL - 14 ER -