TY - JOUR AB - Piperlongumine (PL), a natural product of Piper longum, inhibits multiple malignant phenotypes. Therefore, the present study examined whether PL suppresses cancer stemness in oral cancer. The cellular effects of PL were determined by examining alterations in tumor sphere formation, cell migration, invasion, proliferation ability, chemosensitivity and radiosensitivity. Reverse transcription‑quantitative polymerase chain reaction analysis and western blotting were performed in order to determine molecular expression levels. The present study revealed that PL inhibited cancer stem cell‑forming ability and suppressed the expression of the stemness‑related transcription factors SRY‑Box 2, POU class 5 homeobox 1, and Nanog homeobox. However, it increased the expression of the differentiation marker cytokeratin 18. PL also suppressed cell migration and invasion, resulting in the elimination of the epithelial‑mesenchymal transition. Furthermore, PL increased chemo‑ and radiosensitivity and suppressed tumor growth in vitro and in vivo. The results of the present study suggested that PL inhibits malignant phenotypes via the suppression of cancer stemness in oral cancer. Thus, PL may serve as an effective therapeutic agent for oral cancer. AD - Department of Radiation Oncology, Taipei Medical University Hospital, Taipei 11031, Taiwan, R.O.C. Department of Medical Biotechnology, Medical College, Chang Gung University, Taoyuan 33302, Taiwan, R.O.C. School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, Taiwan, R.O.C. Department of Radiology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan, R.O.C. AU - Chen,Yin‑Ju AU - Kuo,Chia‑Chun AU - Ting,Lai‑Lei AU - Lu,Long‑Sheng AU - Lu,Ya‑Ching AU - Cheng,Ann‑Joy AU - Lin,Yun‑Tien AU - Chen,Chien‑Ho AU - Tsai,Jo‑Ting AU - Chiou,Jeng‑Fong DA - 2018/02/01 DO - 10.3892/ol.2017.7486 EP - 1798 IS - 2 JO - Oncol Lett KW - cancer stem cells epithelial-mesenchymal transition piperlongumine chemosensitivity radiosensitivity oral cancer PY - 2018 SN - 1792-1074 1792-1082 SP - 1789 ST - Piperlongumine inhibits cancer stem cell properties and regulates multiple malignant phenotypes in oral cancer T2 - Oncology Letters TI - Piperlongumine inhibits cancer stem cell properties and regulates multiple malignant phenotypes in oral cancer UR - https://doi.org/10.3892/ol.2017.7486 VL - 15 ER -