TY - JOUR AB - Currently, the underlying mechanism of oxaliplatin (OXA) induced live injury is unclear. In addition, there is no standard clinical treatment for OXA‑induced acute liver injury (ALI). In this study, we established an animal model of OXA‑induced ALI, and studied the role of oxidative stress in OXA‑induced ALI and the impacts of reduced glutathione (GSH) treatment on OXA‑induced ALI. To establish an OXA‑induced ALI model, KM mice received intraperitoneal injection of OXA (8 mg/kg) for 4 days. Serum alanine aminotransferase (ALT), aspartate aminotransferase levels (AST), hepatic pathology and oxidative stress indicators in liver tissues were analyzed. To study the impact of GSH treatment on OXA‑induced ALI, mice were treated with GSH (400 mg/kg, i.p). In this ALI mouse model, ALT and AST levels were significantly increased (P<0.01). Liver pathological examination revealed varying degrees of liver cell turbidity and degeneration, even balloon‑like changes and focal necrosis, and sinusoidal hemorrhage in some cells. Compared with control group, the malondialdehyde (MDA) and GSH levels were significantly increased in OXA‑treated group (P<0.01), while the superoxide dismutase SOD and GSH‑peroxidase levels were decreased after OXA withdrawal (P<0.01). When GSH was used to treat OXA‑induced ALI mice, the pathological injury of liver tissues was alleviated, and serum ALT and AST were significantly decreased. In addition, GSH treatment could reduce the OXA‑induced increase of MDA level (P<0.05) in liver tissues, but had no impact on SOD level (P>0.05). We have successfully established an OXA‑induced ALI model. Using this model, we discover that oxidative stress plays an important role in OXA‑induced ALI. GSH‑based hepatoprotective therapy can partially inhibit oxidative stress and alleviate OXA‑induced ALI. AD - Hepatobiliary Surgery Department, Affiliated Tumor Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China Department of First Chemotherapy, Affiliated Tumor Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China Department of Oncology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China Department of Pathology, Affiliated Tumor Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China Guangxi Liver Cancer Diagnosis and Treatment Engineering and Technology Research Center, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China Chest Hospital of Henan Province, Zhengzhou, Henan 450000, P.R. China AU - Lin,Youzhi AU - Li,Yongqiang AU - Hu,Xiaohua AU - Liu,Zhihui AU - Chen,Jun AU - Lu,Yulei AU - Liu,Juan AU - Liao,Sina AU - Zhang,Yumei AU - Liang,Rong AU - Lin,Yan AU - Li,Qian AU - Liang,Caoyong AU - Yuan,Chunling AU - Liao,Xiaoli DA - 2018/02/01 DO - 10.3892/ol.2017.7594 EP - 2272 IS - 2 JO - Oncol Lett KW - oxaliplatin chemotherapy acute liver injury reduced glutathione oxidative stress hepatoprotective PY - 2018 SN - 1792-1074 1792-1082 SP - 2266 ST - The hepatoprotective role of reduced glutathione and its underlying mechanism in oxaliplatin-induced acute liver injury T2 - Oncology Letters TI - The hepatoprotective role of reduced glutathione and its underlying mechanism in oxaliplatin-induced acute liver injury UR - https://doi.org/10.3892/ol.2017.7594 VL - 15 ER -