TY - JOUR AB - The Ganoderma lucidum (G. lucidum) mushroom is one of the most extensively studied functional foods, known for its numerous health benefits, including the inhibition of tumor cell growth. The present study assessed the anti-proliferative and pro‑apoptotic activity of a novel G. lucidum polysaccharide peptide (GL‑PP) in human glioma U251 cells, which was purified from grass‑cultured G. lucidum. GL‑PP is a glycopeptide with an average molecular weight of 42,635 Da and a polysaccharide‑to‑peptide ratio of 88.70:11.30. The polysaccharides were composed of l‑arabinose, d‑mannose and d‑glucose at a molar ratio of 1.329:0.372:2.953 and a total of 17 amino acids were detected. The results of the current study demonstrated that GL‑PP significantly inhibited U251 cellular proliferation. The proportion of G0/G1 phase cells and sub‑G1 phase cells significantly increased as the concentration of GL‑PP increased, as did the activity of caspase-3. These results indicate that GL‑PP directly inhibited human glioma U251 proliferation by inducing cell cycle arrest and promoting apoptosis. AD - Department of Neurosurgery, The Affiliated Union Hospital of Fujian Medical University, Fuzhou, Fujian 350001, P.R. China National Engineering Research Center of JUNCAO Technology, Fujian Agricultural and Forestry University, Fuzhou, Fujian 350002, P.R. China AU - Wang,Chunhua AU - Lin,Dongmei AU - Chen,Quan AU - Lin,Shuqian AU - Shi,Songsheng AU - Chen,Chunmei DA - 2018/04/01 DO - 10.3892/ol.2018.7823 EP - 4336 IS - 4 JO - Oncol Lett KW - Ganoderma lucidum polysaccharide peptide glioma anti‑proliferation apoptosis PY - 2018 SN - 1792-1074 1792-1082 SP - 4330 ST - Polysaccharide peptide isolated from grass-cultured Ganoderma lucidum induces anti-proliferative and pro-apoptotic effects in the human U251 glioma cell line T2 - Oncology Letters TI - Polysaccharide peptide isolated from grass-cultured Ganoderma lucidum induces anti-proliferative and pro-apoptotic effects in the human U251 glioma cell line UR - https://doi.org/10.3892/ol.2018.7823 VL - 15 ER -