TY - JOUR AB - Phosphoribosyl pyrophosphate synthetase 1 (PRPS1) is closely associated with a number of diseases; however, its influence in B-cell acute lymphoblastic leukemia (B‑ALL) and the potential molecular mechanisms involved remain unclear. The present study aimed to evaluate the expression of PRPS1 in Chinese children with B‑ALL and to investigate the mechanism of action of PRPS1 in this disease. A Cell Counting Kit‑8 (CCK‑8) assay was performed to examine the proliferation of B‑ALL Sup‑B15 and Raji cells, and flow cytometric analysis was conducted to determine the cell cycle distribution and rate of apoptosis. The mRNA and protein expression levels of PRPS1, MYC proto‑oncogene, bHLH transcription factor, cyclin E1, B‑cell lymphoma‑2 (Bcl‑2), cyclin dependent kinase 2 and caspase‑3 were detected by reverse transcription‑quantitative polymerase chain reaction and western blot analysis, respectively. Elevated PRPS1 expression was associated with a high‑risk stratification and poor prognosis in patients with B‑ALL. Furthermore, overexpression of PRPS1 accelerated the growth of and inhibited apoptosis in Sup‑B15 and Raji cells as well as increasing the expression of Bcl‑2 to induce an anti‑apoptotic effect in B‑ALL cell lines. The results of the present study indicate that PRPS1 regulates multiple processes in B‑ALL and may be an attractive therapeutic target. AD - Department of Hematology, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing 400014, P.R. China AU - Ma,Yimei AU - An,Xizhou AU - Guan,Xianmin AU - Kong,Qinglin AU - Wang,Yanzhen AU - Li,Pengfei AU - Meng,Yan AU - Cui,Yinghui AU - Wen,Xianhao AU - Guo,Yuxia AU - Shen,Yali AU - Yu,Jie DA - 2018/04/01 DO - 10.3892/ol.2018.7903 EP - 4322 IS - 4 JO - Oncol Lett KW - B‑cell acute lymphoblastic leukemia phosphoribosyl pyrophosphate synthetase 1 B‑cell lymphoma 2 apoptosis prognosis PY - 2018 SN - 1792-1074 1792-1082 SP - 4314 ST - High expression of PRPS1 induces an anti-apoptotic effect in B-ALL cell lines and predicts an adverse prognosis in Chinese children with B‑ALL T2 - Oncology Letters TI - High expression of PRPS1 induces an anti-apoptotic effect in B-ALL cell lines and predicts an adverse prognosis in Chinese children with B‑ALL UR - https://doi.org/10.3892/ol.2018.7903 VL - 15 ER -