TY - JOUR AB - Blocking the activation of nuclear factor κB (NF-κB) is a promising strategy for the treatment of non-small cell lung cancer. The circumsporozoite protein (CSP), a key component of the sporozoite stage of the malaria parasite, was previously reported to block NF‑κB activation in hepatocytes. Therefore, in the present study, the effect of CSP on the growth of the human lung cancer cell line, A549, was investigated. It was demonstrated that transfection with a recombinant plasmid expressing CSP was able to inhibit the proliferation of A549 cells in a dose‑dependent manner and induce the apoptosis of A549 cells. A NF‑κB gene reporter assay indicated that CSP and its nuclear localization signal (NLS) motif were able to equally suppress the activation of NF‑κB following stimulation with human recombinant tumor necrosis factor (TNF)‑α in A549 cells. Furthermore, western blot analysis indicated that NLS did not affect the phosphorylation and degradation of IκB, but was able to markedly inhibit the nuclear translocation of NF-κB in TNF-α stimulated A549 cells. Therefore, the data suggest that CSP may be investigated as a potential novel NF‑κB inhibitor for the treatment of lung cancer. AD - Department of Thoracic Surgery, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, P.R. China Institute of Immunology of PLA, College of Basic Medical Sciences, The Third Military Medical University, Chongqing 400038, P.R. China Department of Pathogenic Biology, College of Basic Medical Sciences, The Third Military Medical University, Chongqing 400038, P.R. China AU - Deng,Xu‑Feng AU - Zhou,Dong AU - Liu,Quan‑Xing AU - Zheng,Hong AU - Ding,Yan AU - Xu,Wen‑Yue AU - Min,Jia‑Xin AU - Dai,Ji‑Gang DA - 2018/05/01 DO - 10.3892/ol.2018.8115 EP - 6591 IS - 5 JO - Oncol Lett KW - circumsporozoite protein nuclear transcription factor κb human lung cancer cell line proliferation apoptosis PY - 2018 SN - 1792-1074 1792-1082 SP - 6585 ST - Plasmodium circumsporozoite protein suppresses the growth of A549 cells via inhibiting nuclear transcription factor κB T2 - Oncology Letters TI - Plasmodium circumsporozoite protein suppresses the growth of A549 cells via inhibiting nuclear transcription factor κB UR - https://doi.org/10.3892/ol.2018.8115 VL - 15 ER -