TY - JOUR AB - Embelin, as an inhibitor of the X-linked inhibitor of apoptosis protein (XIAP), may induce apoptosis in various types of cancer cells. The present study aimed to determine the effect of Embelin on the tumor necrosis factor‑related apoptosis‑inducing ligand (TRAIL)‑induced apoptosis of osteosarcoma cells. Embelin and TRAIL were applied to U2OS and MG63 cells, respectively or in combination. MTT was initially used to detect the difference in survival rates between the group receiving combined application of 100 ng/ml TRAIL and 20 µmol/l Embelin and the individual application groups. Light microscopic quantification was used to detect the morphology of the osteosarcoma cells in each group. Determination of cell apoptosis was subsequently performed using flow cytometry. The invasive ability of the cells was detected by a Transwell assay, prior to relative protein expression being determined by western blot analysis. Based on all the test data, it was revealed that the survival rates and the invasive ability were significantly lower following the combined application of 100 ng/ml TRAIL and 20 µmol/l Embelin than following the individual application of either (P<0.01). Additionally, upregulating expression of caspases, as well as death receptor 5, and downregulating expression of XIAP and matrix metalloproteinase 9 (MMP‑9), had more significant effects in the combined group compared with the individual group and the control group. All these results suggested that Embelin may enhance TRAIL‑induced apoptosis and inhibit the invasion of human osteosarcoma cells. AD - Department of Orthopedics, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA AU - Qian,Hao AU - Chen,Yao AU - Huang,Tao AU - Liu,Tiemin AU - Li,Xiucheng AU - Jiang,Guangjian AU - Zhang,Wei AU - Cheng,Shuo AU - Li,Pengcheng DA - 2018/05/01 DO - 10.3892/ol.2018.8209 EP - 6940 IS - 5 JO - Oncol Lett KW - osteosarcoma Embelin TRAIL apoptosis invasion PY - 2018 SN - 1792-1074 1792-1082 SP - 6931 ST - Combined application of Embelin and tumor necrosis factor-related apoptosis-inducing ligand inhibits proliferation and invasion in osteosarcoma cells via caspase-induced apoptosis T2 - Oncology Letters TI - Combined application of Embelin and tumor necrosis factor-related apoptosis-inducing ligand inhibits proliferation and invasion in osteosarcoma cells via caspase-induced apoptosis UR - https://doi.org/10.3892/ol.2018.8209 VL - 15 ER -