TY - JOUR AB - DNA methylation is closely associated with aberrant epigenetic changes. Previous studies have identified various genes associated with non‑small cell lung cancer (NSCLC), but the precise combination responsible for its etiology is still debated. The aim of the present study was to select a new set of NSCLC‑related genes using methylation‑sensitive high‑resolution melting. The promoter methylation status of six selected genes, consisting of protocadherin γ subfamily B, 6 (PCDHGB6), homeobox A9 (HOXA9), O6‑methylguanine‑DNA methyltransferase (MGMT), microRNA (miR)‑126, suppressor of cytokine signaling 3 (SOCS3) and Ras association domain family member 5, also termed NORE1A, was evaluated in 54 NSCLC patients. From these samples, genome‑wide DNA was extracted and bisulfite conversion was performed along with fluorogenic quantitative polymerase chain reaction to detect methylation values of the six selected promoters. The present results revealed frequent methylation on PCDHGB6, HOXA9 and miR‑126, which contrasted with infrequent methylation on MGMT. The results indicated no methylation on either SOCS3 or NORE1A. The sensitivity and specificity of the methylation assessment were 85.2 and 81.5%, respectively, and the analysis results were validated by pyrosequencing. Furthermore, minute comparison of the association between DNA methylation and clinical features was performed. Overall, these results may provide potential information for the development of better clinical diagnostics and more targeted and effective therapies for NSCLC. AD - State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, Shanghai 200050, P.R. China Department of Pathology, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, Fujian 350002, P.R. China AU - Liu,Fangming AU - Zhang,Honglian AU - Lu,Shaohua AU - Wu,Zhenhua AU - Zhou,Lin AU - Cheng,Zule AU - Bai,Yanan AU - Zhao,Jianlong AU - Zhang,Qiqing AU - Mao,Hongju DA - 2018/05/01 DO - 10.3892/ol.2018.8321 EP - 7648 IS - 5 JO - Oncol Lett KW - methylation high‑resolution melting CpG lung cancer diagnosis PY - 2018 SN - 1792-1074 1792-1082 SP - 7639 ST - Quantitative assessment of gene promoter methylation in non‑small cell lung cancer using methylation‑sensitive high‑resolution melting T2 - Oncology Letters TI - Quantitative assessment of gene promoter methylation in non‑small cell lung cancer using methylation‑sensitive high‑resolution melting UR - https://doi.org/10.3892/ol.2018.8321 VL - 15 ER -